针刀松解联合丹参注射液关节腔注射治疗骨性关节炎模型兔

文题释义： 针刀疗法：用一个完整的语言来概括应该是一种介于手术方法和非手术疗法之间的闭合性松解术。针刀疗法是将东方中医学的基本理论和西方医学的手术解剖基本理论融为一体，针刀疗法其操作的特点是在治疗部位刺入深部到病变处进行轻松的切割、剥离等不同的刺激，以达到止痛祛病的目的。 丹参注射液：其化学成分主要是水溶性酚酸类化合物，通过多种靶标起到促进血液循环和祛瘀的作用。动物研究表明，丹参注射液可通过抑制IκBα磷酸化来抑制NF-κB信号通路的激活。保护软骨下骨及软骨细胞，有效防治骨关节炎。 背景：丹参注射液与针刀松解术都是临床中治疗骨关节炎很常用的技术，目前缺乏对2种治疗方法联合使用的研究。 目的：对比单独使用针刀松解术、单独使用丹参注射液与两种方法联合使用治疗兔模型骨关节炎的效果。 方法：实验方案经黑龙江中医药大学动物实验伦理委员会批准。50只新西兰白兔分为5组：空白对照组；模型组；丹参注射液组；针刀组；针刀联合丹参注射液组。除空白对照组外，其他4组兔均制备膝关节骨关节炎模型。造模成功后，对针刀组兔内外侧支持带、内外侧膝眼，膝关节上方髌上囊、股四头肌各肌肌腹，膝关节内侧股薄肌肌腹及止点、收肌肌群肌腹及止点和胫骨平台附近部位进行针刀松解；空白对照组及模型组兔关节腔注射生理盐水0.3 mL；丹参注射液组兔关节腔每次注射0.3 mL丹参注射液；针刀联合丹参注射液组：每周进行1次针刀治疗同针刀组，每次针刀干预之后，关节腔注0.3 mL丹参注射液。上述干预每周1次，持续5周。对各组兔膝关节软骨进行大体观察及病理切片组织学观察，ELISA 检测关节液白细胞介素1、肿瘤坏死因子α水平。 结果与结论：①大体观察：模型组膝关节磨损较为严重，针刀组、丹参注射液组、针刀联合丹参注射液组膝关节轻度磨损；②病理切片评分：丹参注射液组评分略高于针刀组和针刀联合丹参注射液组差异无显著性意义；③ELISA 检测结果：针刀联合丹参注射液组白细胞介素1、肿瘤坏死因子α水平显著低于或针刀组或丹参注射液组(P < 0.05)；④结果说明，在兔膝关节骨关节炎模型中降低白细胞介素1、肿瘤坏死因子α水平方面，针刀松解术结合丹参注射液关节腔注射治优于单独使用针刀或单独使用丹参注射液关节腔注射。ORCID: 0000-0003-4119-633X(刘广宇) 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Needle-knife therapy combined with intra-articular injection of salvia injection for treating osteoarthritis in rabbit models

BACKGROUND:Salvia injection and needle-knife therapy are commonly used techniques in the treatment of osteoarthritis in clinical practice.At present,there is no research on the combination of the two methods.OBJECTIVE:To compare the effects of needle-knife therapy and salvia injection and their combinations in the treatment of osteoarthritis in rabbit models.METHODS:The study was approved by the Laboratory Animal Ethical Committee of Heilongjiang University of Chinese Medicine.Fifty New Zealand white rabbits were divided into five groups:blank control,model,salvia injection,needle-knife,and combination groups.The rat models of osteoarthritis were established in all groups except for blank control group.After successful modeling,medial and lateral patellar retinaculums,medial and lateral knee eyes,suprapatellar bursa,muscle belly of each quadriceps femoris,muscle belly and ending point of gracilis,muscle belly and ending point of adductor and area surrounding the tibial plateau of rats in the needle-knife group received needle-knife release.The rabbits in the blank control and model groups underwent the intra-articular injection of normal saline(0.3 mL).The rabbits in the salvia injection group were given the intra-articular injection of salvia(0.3 mL).The rabbits in the combination group received needle-knife release,followed by intra-articular injection of salvia(0.3 mL).All interventions were conducted weekly,for 5 consecutive weeks.The knee articular cartilage underwent gross observation and histological observation.The levels of interleukin-1 and tumor necrosis factor-alpha in synovial fluid were detected by ELISA.RESULTS AND CONCLUSION:(1)Gross observation:the worn artificial knee in the model group was serious,and was slight in the salvia injection,needle-knife,and combination groups.(2)Pathological scores:the scores in the salvia injection group were slightly higher than those in the needle-knife and combination groups.(3)ELISA results:the levels of interleukin-1 and tumor necrosis factor-alpha in the combination group were significantly lower than those in the salvia injection and needle-knife groups(P<0.05).(4)In summary,needle-knife therapy combined with intra-articular injection of salvia is superior to needle-knife therapy alone or intra-articular injection of salvia in reducing the levels of interleukin-1 and tumor necrosis factor-alpha in rabbit models of osteoarthritis.