玉米黄质对高脂诱发鹌鹑模型血管脂质过氧化损伤的保护作用

背景：大多数人工合成的抗氧化药物长期应用均有一定的毒副作用,玉米黄质作为天然药物有其独特的生理效应。 目的：观察玉米黄质对高脂诱发血管脂质过氧化损伤效应鹌鹑模型影响的量效关系。 方法：建立鹌鹑高脂模型,利用玉米黄质按30,60mg/kg灌胃7周后,取空腹血测定血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇及丙二醛水平、血清超氧化物歧化酶、谷胱甘肽过氧化物酶及诱导型一氧化氮合酶活性;检测各组动物肝脏组织中总胆固醇、三酰甘油水平;并采用苏木精-伊红染色观察主动脉血管病理变化。 结果与结论：与高脂模型组相比,玉米黄质组血清三酰甘油、总胆固醇和低密度脂蛋白胆固醇浓度明显降低,而血清高密度脂蛋白胆固醇浓度明显增高;肝脏总三酰甘油及总胆固醇的合成也受到强烈抑制;玉米黄质组血清超氧化物歧化酶活性、谷胱甘肽过氧化物酶活性明显提高,而诱导型一氧化氮合酶活性下降、丙二醛水平减少。说明玉米黄质具有良好的降脂功效,对高脂诱导的血管脂质过氧化损伤有明显的抑制作用,且高剂量较低剂量作用效果更为理想。

Protective effect of zeaxanthin on lipid peroxidation injure of blood vessel induced by high lipid in quail model

BACKGROUND:Most synthetic long-term use of antioxidant drugs have certain side effects, zeaxanthin as a natural medicine has its unique physiological effects. OBJECTIVE:To observe the dose-effect relationship of zeaxanthin on lipid peroxidation injure of blood vessel induced by high lipid in quail hyperlipidmia model. METHODS:Quail hyperipidmia models were established. A total of 40 quails were randomly divided into four groups: control group, model group, zeaxanthin low-dose group and zeaxanthin high-dose group. Seven weeks after gavage using zeaxanthin with 30, 60mg/kg, fasting plasma was harvested to determine serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA) level, serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and activity of inducible nitric oxide synthase (iNOS). The TC and TG levels of liver tissue in each group were detected; aortic vascular pathological changes were observed with hematoxylin - eosin staining. RESULTS AND CONCLUSION:Compared with model group,high-dose group of zeaxanthin of the concentration of serum TG, TC, and LDL-C were significantly decreased, while the concentration of serum HDL-C were significantly increased. The synthesis of liver total TG and TC was also strongly inhibited. The activity of glutathione peroxidase and supervxide dismutase were significantly increased, however, the activity of inducible nitric oxide synthase was decreased and MDA level was reduced. Zeaxanthin has a good lipid-lowing effect, and plays a notable inhibitory role in lipid peroxidation injure of blood vessel induced by high lipid. In addition, high-dose of zeaxanthin has better effects than low-dose.