紫草素对人膀胱癌T24细胞增殖、凋亡、迁移与侵袭的影响

目的探讨紫草素对人膀胱癌T24细胞增殖、凋亡、迁移与侵袭的影响及可能机制。方法将人膀胱癌T24细胞分为空白对照组与紫草素组(10、20、40μmol/L)。不同浓度紫草素干预48h后,MTT方法检测T24细胞的增殖活力,流式细胞术检测T24细胞的凋亡率。40μmol/L紫草素干预48 h后,Transwell实验检测紫草素对T24细胞迁移与侵袭能力的影响;Western blot实验检测T24细胞中半胱氨酸天冬氨酸蛋白水解酶3/9(Caspase-3/Caspase-9)与基质金属蛋白酶2/9(MMP-2/MMP-9)的表达。结果不同浓度紫草素均能显著抑制T24细胞增殖,并促进细胞凋亡,且呈浓度依赖性(P<0.01)。40μmol/L紫草素干预48 h后,T24细胞迁移与侵袭能力显著降低,T24细胞中Caspase-3、Caspase-9、MMP-2与MMP-9的蛋白表达水平均显著升高(P<0.01)。结论紫草素能够抑制膀胱癌T24细胞增殖、迁移与侵袭,并促进凋亡。

Effects of shikonin on proliferation,apoptosis,migration and invasion of human bladder cancer T24 cells

Objective To investigate the effect of shikonin on proliferation,apoptosis,migration and invasion of human bladder cancer T24 cells and its possible mechanism.Methods T24 cells were divided into control group and shikonin group(10,20,40μmol/L).The proliferation of T24 cells was detected by MTT and the apoptosis rate of T24 cells was detected by flow cytometry 48 hours after shikonin intervention.After 40μmol/L shikonin intervention for 48 hours,Transwell test was used to detect the effect of shikonin on the migration and invasion of T24 cells;Western blot test was used to detect the expression of Caspase-3/caspase-9 and MMP-2/MMP-9 in T24 cells.Results Different concentrations of shikonin can significantly inhibit the proliferation of T24 cells and promote cell apoptosis,which is concentration dependent(P<0.01).After treatment with shikonin at 40μmol/L for 48 hours,the migration and invasion ability of T24 cells was decreased significantly,and the expression of Caspase-3,caspase-9,MMP-2 and MMP-9 in T24 cells was increased significantly(P<0.01).Conclusion Shikonin can inhibit the proliferation,migration and invasion of T24 cells,and promote apoptosis.