| 胃复康对人SGC-7901胃癌细胞株p53、bcl-2基因表达的影响 |
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| 引用本文： | 蔡朔,刘山,王旭,蔡玉文.胃复康对人SGC-7901胃癌细胞株p53、bcl-2基因表达的影响[J].解剖科学进展,2009,15(1):50-52. |
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| 作者姓名： | 蔡朔 刘山 王旭 蔡玉文 |
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| 作者单位： | 1. 辽宁中医药大学,教学实验中心基础医学院
2. 沈阳市第四人民医院,辽宁,沈阳,110032 |
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| 摘 要： | 目的探讨中药胃复康对人SGC-7901胃癌细胞株p53、bcl-2基因表达的影响。方法PRMI1640血清培养的人SGC-7901胃癌细胞株分为胃复康实验组、生理盐水对照组和顺铂(DDP)化疗组。利用血清药理学方法制备药物血清,作用48h后,应用免疫细胞化学方法和图像分析方法观察胃复康、DDP对人SGC-7901胃癌细胞株p53、bcl-2基因表达影响。结果免疫细胞化学法显示抑癌基因p53和癌基因bcl-2主要定位在细胞质内。胃复康、DDP药物血清作用后,p53表达明显增强,bcl-2表达明显降低;随着作用时间延长,两种基因表达改变更明显。胃复康、DDP药物血清组p53和bcl-2表达的光密度值(AOD)与对照组差异显著(P<0.01)。结论中药胃复康药物血清上调p53和下调bcl-2的表达可能是其抑制胃癌细胞株SGC-7901的作用机制之一。
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| 关 键 词： | 中药胃复康 人SGC-7901胃癌细胞株 p53 bcl-2 |
The Alteration of Expression of p53 and bcl-2 by Wei-Fu-Kang in the Human SGC-7901 Stomach Cancer Cells |
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| CAI Shuo,LIU Shan,WANG Xu,CAI Yu-wen.The Alteration of Expression of p53 and bcl-2 by Wei-Fu-Kang in the Human SGC-7901 Stomach Cancer Cells[J].Progress of Anatomical Sciences,2009,15(1):50-52. |
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| Authors: | CAI Shuo LIU Shan WANG Xu CAI Yu-wen |
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| Institution: | Department of Anatomy and Histoembryology;Liaoning University of Traditional Chinese Medicine;Shenyang 110032 China |
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| Abstract: | Objective To investigate the alteration of expression of p53 and bcl-2 by Wei-Fu-Kang in the human SGC-7901 stomach cancer cells.Methods Human SGC-7901 stomach cancer cells cultured in PRMI1640 were divided into 3 groups:wei-fu-kang treating group,saline control group and cisplatin chemiotherapy(DDP) group.48 hours later,immunohistochemical techniques and microimage analysis system were used to observe and analyze the expression of p53 and bcl-2.Results The positive p53 and bcl-2 products were localized in ... |
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| Keywords: | p53 bcl-2 |
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