脂质体介导的VEGF质粒对成年大鼠脑缺血再灌注后大脑皮质内P-MeCP2表达的影响

目的观察脂质体介导的VEGF质粒对成年大鼠脑缺血再灌注后大脑神经元phosphorylation of methyl-CpG binding protein 2 (P-MeCP2)表达的影响,探讨VEGF促进缺血损伤后神经元新生的可能机制,进一步为VEGF治疗缺血性脑中风提供实验和理论依据。方法 30只SD大鼠,随机分为假手术组(sham组)、质粒组(VEGF组)和对照质粒组(vehicle组),采用左侧大脑中动脉线栓(MCAO)模型,侧脑室给药,免疫印迹、免疫荧光三标染色及激光共聚焦扫描技术等方法检测P-MeCP2的表达。结果再灌2周VEGF质粒组P-MeCP2、BrdU和NeuN同时表达于缺血侧大脑皮质神经元,而缺血对侧没有找到,VEGF质粒组皮质内P-MeCP2的表达较对照组、假手术组显著增高(p<0.01)。结论脂质体介导的VEGF质粒能促进成年大鼠脑缺血神经元的新生,可能与上调P-MeCP2的表达有关。

Influence of liposome-mediated VEGF plasmid on the expression of P-MeCP2 in cortex of the adult rats after brain ischemia and reperfusion injury

Objective To observe the influence of liposome-mediated VEGF plasmid on the expression of PMeCP2 in cortex of the adult rats after brain ischemia and reperfusion injury.Methods Thirty Sprague-Dawley rats were assigned randomly into a VEGF group(experimental group),a sham group(placebo group) and a vehicle group(control group).Middle cerebral artery occlusion(MCAO) was applied as cerebral ischemia model.Liposome-mediated VEGF plasmid was injected into lateral ventricle,western blotting,triple immunofluorescence staining and confocal laser scanning techniques were utilized to examine the expression of P-MeCP2.Results Co-localizing of P-MeCP2 with BrdU and NeuN was found in cerebral cortex neurons of ischemic hemisphere,but not found in the opposite side in 2w VEGF group.The expression level of P-MeCP2 was significantly higher in 2w VEGF group than in sham control group and the placebo group(p<0.01).Conclusion Neogenesis of liposome-mediated VEGF plasmid might be related to upregulation of P-MeCP2 expression in brain ischemia and reperfusion injury rats.