| 胎鼠海马神经元原代培养及转染条件的优化 |
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| 引用本文： | 张勇,张国宁,丛敬,倪月秋.胎鼠海马神经元原代培养及转染条件的优化[J].解剖科学进展,2011,17(5):464-467. |
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| 作者姓名： | 张勇 张国宁 丛敬 倪月秋 |
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| 作者单位： | 1. 沈阳医学院基础医学院组织与胚胎学教研室,辽宁,沈阳,110034
2. 中国医科大学93期医学影像系 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 建立一种稳定的大鼠胎鼠海马神经元培养方法并能够有效的鉴定其纯度,探讨用化学转染试剂体外转染大鼠胎鼠海马神经元的最佳转染条件.方法 分离Wista大鼠E18d胚胎并取海马组织,无血清培养基培养并做MAP2荧光染色鉴定其纯度.应用lipofectamine <'TM>2000转染试剂盒,大鼠神经元电转试剂盒,lipo...
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| 关 键 词： | 海马神经元 原代培养方法 转染 优化 大鼠 |
Primary culture and the transfection conditions optimization of hippocampal neurons in fetal rat |
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| ZHANG Yong,ZHANG Guo-ning,CONG Jing,NI Yue-qiu.Primary culture and the transfection conditions optimization of hippocampal neurons in fetal rat[J].Progress of Anatomical Sciences,2011,17(5):464-467. |
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| Authors: | ZHANG Yong ZHANG Guo-ning CONG Jing NI Yue-qiu |
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| Institution: | ZHANG Yong1*,ZHANG Guo-ning2,CONG Jing1,NI Yue-qiu1 (1. Histology and Embryology Department,Shenyang Medical College,Liaoning Shenyang 110034,2. Department of Medical Imaging,China Medical University,Liaoning Shenyang 110001 China) |
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| Abstract: | Objective To select a suitable primary culturing method for hippocampus neurons of newborn Wista rats and to discuss the best condition of chemical transfection agents for transfection on hippocampus neuron. Methods The hippocampus of embryo 18 d Wista rats was dissected out and the neurons were cultured and transfected by lipofectamineTM 2000, electric transfection reagents and lipofectamine TM LTX respectively. Expression of green fluorescent protein was observed and the survival rate of hippocampus neuro... |
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| Keywords: | hippocampus neurons primary culture transfection optimization rat |
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