人乳腺癌组织中CD1C+树突状细胞免疫组化研究

目的观察树突状细胞在人乳腺癌组织中的形态学变化。方法利用CD1C+作为树突状细胞(dendriticcell,DC)的特征性标志分子,用免疫组织化学方法观察20例人乳腺癌组织及癌旁相对正常组织中树突状细胞的分布和形态学变化,同时检测p53蛋白的表达。结果乳癌组织中树突状细胞CD1C+阳性面数密度(4.55±1.32)和平均光密度值(0.13±0.03)明显低正常乳腺组织面数密度(11.16±5.32)和平均光密度值(0.27±0.07,P<0.05);乳癌组织DC数量较正常乳腺组织明显减少(P<0.05)。20例乳腺癌中,p53蛋白阳性表达率为60%;正常组织为阴性。结论树突状细胞及其表达的p53蛋白可能参与肿瘤免疫调节作用。

Immunohistochemical Study of Dendritic Cells in Human Breast Cancer

Objectives To observe the immunohistochemical changes of dendritic cells(DCs) in human breast cancer.Methods The distribution and morphological changes of DCs in 20 cases of human breast cancer were observed with immunohistochemitry,using CD 1C+ monoclonal antibody as specific marker for DCs,and the expression of p53 was determined also.Results The square density (SD, 4.55±1.32) and mean optic density (MOD, 0.13±0.03) of CD 1C+ positive product in DCs of human breast cancer were lower than those (11.16±5.32, 0.27± 0.07) in normal breast tissue respectively (P<0.05),and the number of DCs declined greatly in breast cancer tissue compared with normal breast tissue. The positive expression rate of p53 protein was 60% in 20 cases of breast cancer thssue,but negative in normal breast tissue.Conclusion DCs and p53 protine may play an important regulatory role in initiating immune response against breast cancer.