| 糖尿病肾病肾组织中基质金属蛋白酶-9表达的定量分析 |
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| 引用本文： | 吴志山,陶仕英,张露,刘萍.糖尿病肾病肾组织中基质金属蛋白酶-9表达的定量分析[J].解剖科学进展,2008,14(1):9-11. |
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| 作者姓名： | 吴志山 陶仕英 张露 刘萍 |
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| 作者单位： | 1. 辽宁医学院2005级24班,辽宁,锦州,121000
2. 辽宁医学院组织胚胎学教研室,辽宁,锦州,121000 |
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| 基金项目： | 辽宁医学院大学生科技创新课题 |
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| 摘 要： | 目的对糖尿病肾病(DN)大鼠模型的肾组织中的基质金属蛋白酶-9(MMP-9)的表达进行定量研究。方法取成年健康SD大鼠20只,随机分成糖尿病组(DM组)和正常对照组(NC组),DM组大鼠采用一次性腹腔内注射STZ诱导制造糖尿病大鼠模型。分别于造模后第4、8周行HE染色,在光镜下观察肾小球大小和肾小球内细胞数目的变化。通过免疫组织化学方法观察肾脏局部MMP-9的表达,以MMP-9平均光密度做为观察指标。结果HE染色:DM组8周时见肾小球体积增大,肾小球内系膜区增宽.细胞核数目增加。免疫组织化学:DM组大鼠肾脏MMP-9的表达明显低于正常大鼠(P<0.05)。结论糖尿病肾病时MMP-9表达水平降低,可能是引起肾小球细胞外基质积聚和降解减少的重要原因之一。
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| 关 键 词： | 糖尿病肾病 基质金属蛋白酶-9 大鼠 |
| 文章编号： | 1006-2947（2008）01-0009-03 |
| 收稿时间： | 2007-06-29 |
| 修稿时间： | 2007年6月29日 |
The Quantitative Analysis of MMP-9 in Kidney of Diabetic Rats |
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| WU Zhi-shan,TAO Shi-yin,ZHANG Lu,LIU Ping.The Quantitative Analysis of MMP-9 in Kidney of Diabetic Rats[J].Progress of Anatomical Sciences,2008,14(1):9-11. |
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| Authors: | WU Zhi-shan TAO Shi-yin ZHANG Lu LIU Ping |
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| Abstract: | Objective To investigate the expression of matrix metalloproteinase-9(MMP-9)in kidney of diabetic rats.Methods 20 adult SD rats were randomly divided into normal control group(NC)and diabetic model group(DM).After diabetic model rats were induced by streptozotocin(STZ),the morphological changes were observed through the light microscope and immunohistochemical staining was employed to detect the expression of MMP-9 in kidney and analyzed with image analysis system.Results The morphological changes of the glomeluri of diabetic rats were obvious,but with the normal structure of the normal rats.The expression of MMP-9 in diabetic kidneys was significantly lower than that in normal control rats(P<0.05).Conclusion Downexpression of MMP-9 in renal glomeluri in diabetic rats may promote the accumulation of extracellular matrix in kidney. |
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| Keywords: | diabetic nephropathy matrix metalloproteinase-9 rat |
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