Identification of Malassezia species isolated from some Malassezia associated skin diseases |
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| Authors: | Mervat Hamdino Amany Ahmed Saudy Laila Hussein El-Shahed Mohamed Taha |
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| Institution: | 1. Dermatology and Venereology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt;2. Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt;6. Department of Pathology, Basavatarakam Indo American Cancer Hospital & Research Institute, Hyderabad, India;1. Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India;2. Advanced Eye Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India;3. Precise Healthcare Clinical Laboratory, Sector 30, Chandigarh;1. Department of Bacteriology, University Hospital of Dijon, BP 37013, 21070 Dijon CEDEX, France;2. UMR/CNRS 6249 Chrono-environnement, University of Bourgogne-Franche-Comté, Besançon, France;3. Laboratoire de Parasitologie-Mycologie, Plateforme de Biologie Hospitalo-universitaire, 2 rue A. Ducoudray, BP 37013, 21070 Dijon Cedex, France;4. UMR PAM Univ Bourgogne Franche-Comté - AgroSup Dijon - Equipe Vin, Aliment, Microbiologie, Stress, 21078 Dijon, cedex, France;1. Uluda? University Faculty of Medicine, Department of Pulmonology, Bursa, Turkey;2. Uluda? University Faculty of Medicine, Department of Internal Medicine, Bursa, Turkey;3. Uluda? University Faculty of Medicine, Department of Hematology, Bursa, Turkey;4. Uluda? University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Bursa, Turkey;5. Uluda? University Faculty of Medicine, Department of Microbiology, Bursa, Turkey |
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| Abstract: | BackgroundThe genus Malassezia represents the dominant eukaryotic component of the skin microbial flora. There are complex interactions between this commensal and the skin, leading to various Malassezia-caused or Malassezia exacerbated skin conditions.ObjectivesTo identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.MethodsScrapings were collected from 80 patients (40 PV, 20 AD, and 20 SD) and 30 healthy subjects. For 10-14 days, specimens were cultured on Dixon's medium and Malt extract agar. Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.ResultsThe isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis. In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%). Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%). Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).ConclusionsMalassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions. Chrome agar media can be promising for the identification of Malassezia species phenotypically. However, species differentiation has to be complemented by molecular methods. |
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