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ES细胞源性表皮干细胞在皮肤缺损创面的分化
引用本文:黄锦桃,刘爱军,朱永红,李海标.ES细胞源性表皮干细胞在皮肤缺损创面的分化[J].解剖学研究,2007,29(1):26-28,F0004.
作者姓名:黄锦桃  刘爱军  朱永红  李海标
作者单位:中山大学中山医学院组织与胚胎学教研室,广东,广州,510080
基金项目:国家重点基础研究发展计划(973计划)
摘    要:目的探讨ES细胞源性表皮干细胞移植入小鼠全层皮肤缺损后对创面的修复作用,为组织工程皮肤的研究提供新的思路。方法将羊膜诱导后的带有核荧光标记的ES细胞源性表皮干细胞以生物膜为载体,覆盖小鼠全层皮肤缺损创面。术后1~8周连续取材,HE染色观察,苏丹Ⅲ染色,β1整合素、CK15、CK19、CEA免疫组化荧光双标观察。结果术后2周,创面完全长合,较厚的新生皮完全覆盖创面,基底层细胞增生,形成细胞柱伸向真皮层,真皮层中可见管腔样结构,4周后新生表皮开始变薄,新生表皮下可见毛囊样、汗腺样、皮脂腺样等结构,皮脂腺样结构苏丹Ⅲ染色阳性。免疫双标结果显示,1~4周新生表皮基底层细胞呈β1整合素、CK19阳性,真皮层中带有核标记的管状或泡状结构呈CK15、CEA阳性。结论ES细胞源性表皮干细胞在小鼠全层皮肤缺损创面可分化为表皮样、汗腺样、毛囊样和皮脂腺样等结构的潜能。

关 键 词:胚胎干细胞  表皮干细胞  定向分化  皮肤附属结构  组织工程
收稿时间:2006-09-20
修稿时间:2006-09-20

Differention of ES cell-derived epidermal stem cells in full thickness skin defects
HUANG Jin-tao,LIU Ai-jun,ZHU Yong-hong,LI Hai-biao.Differention of ES cell-derived epidermal stem cells in full thickness skin defects[J].Anatomy Research,2007,29(1):26-28,F0004.
Authors:HUANG Jin-tao  LIU Ai-jun  ZHU Yong-hong  LI Hai-biao
Institution:Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 China
Abstract:Objective To investigate the healing effects of ES cell-derived epidermal stem cells in full thickness skin defects. Methods Epidermal stem cells, labeled with Hoechst 33342, carried by a layer of biomembrane, were transplanted into mice full thickness skin defects. The differentiation tissue of donor cells was sampled each week. The sections were observed with HE staining, Sudan Ⅲ staining, immunohistochemical method and bi-labeled immunofluorescence methods to test the expression of β1 integrin, CK15, CK19, and CEA. Results The full thickness skin defects were healed 2 weeks after the operation. Newborn skin was thicker than the normal. The basal cells were proliferated and formed bulky cellular poles towards dermis. There were tubular or follicular structures in dermis. There were sweat glands-like, sebaceous glands-like and hair follicles-like structures in new-born skin after 4 weeks. The sebaceous gland-like structure showed orange by Sudan Ⅲ staining. The basal cells of keratinized stratified squamous epithelium expressed β1 integrin and CK19 bi-labeled immunofluorescence positive respectively. Cells labeled by Hoechst 33342, located in the epidermis and tubular or follicular structures in dermis, expressed CK15 and CEA positive respectively. Conclusion ES cell-derived epidermal-like stem cells can differentiate epidermis, sweat glands-like, sebaceous glands-like and hair follicles-like structures in newborn skin.
Keywords:Embryonic stem cells  Epidermal stem cells  Committed differentiation  Skin appendages  Tissue engineering
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