中国部分城市A组轮状病毒感染状况调查及VP7血清型分析

目的 研究我国A组轮状病毒感染状况及VP7血清型分布。方法 利用PAGE电泳检测北京、沈阳、新乡、上海、深圳、广州六个城市1997和（或）1998年秋冬季婴幼儿腹泻A组轮状病毒的感染状况，并采用RT－PCR方法对4种主要的VP7血清型（G1、G2、G3和G4型）进行分型。结果 374份标本中有181份为轮状病毒阳性，检出率48．4％。各地标本的检出率为32％－63％之间，北京、上海地区两年的检出率基本近似。阳性标本经PCR分型方法鉴定G1、G2、G3和G4型的感染率分别为89．5％、6．1％、2．8％和0。有两例为G1和G2型混合感染，另有1例经测序证实为G9型。各地之间、每年之间RV的流行状况略有差异。结论 G1、G2、和G3型是我国A组轮状病毒感染的主要血清型。

Investigation of group A rotavirus infection in several cities in China and prevalence of VP7 serotype

OBJECTIVE: To study the prevalence of Group A rotavirus infection in China. METHODS: 374 samples from infants with diarrhea were collected from six cities (Beijing, Shenyang, Xinxiang, Shanghai, Shenzhen and Guangzhou) in autumn and winters of 1997 and/or 1998. The incidence of rotavirus infection and the prevalence of the four dominant serotypes G1, G2, G3, G4(VP7 serotype) were detected by PAGE and RT-PCR. RESULTS: Of the 374 samples detected, 181 were rotavirus positive. The positive rate was 48.4%, ranging from 32%-63% in different cities. The positive rates between 1997 and 1998 in Beijing and Shanghai were similar. Serotyped by RT-PCR method based on VP7 sequence, the positive rates of type G1, G2, G3 and G4 was 89.5%, 6.1%, 2.8% and 0, respectively. Two cases were found being coinfected with G1 and G2, and one case was confirmed to be infected with G9 by cDNA sequence analysis. There was a little difference in prevalence of G type rotavirus infection among samples from different cities and different years. CONCLUSION: The results indicated that rotavirus was the most important etiologic agent in this country, and the predominant serotypes were G1, G2 and G3.