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2004-2005年中国B型流感病毒抗原性及基因特性研究
引用本文:张烨,温乐英,赵翔,李梓,郭俊峰,徐翠玲,王敏,余宏杰,杨维中,郭元吉,舒跃龙.2004-2005年中国B型流感病毒抗原性及基因特性研究[J].中华实验和临床病毒学杂志,2006,20(2):11-13.
作者姓名:张烨  温乐英  赵翔  李梓  郭俊峰  徐翠玲  王敏  余宏杰  杨维中  郭元吉  舒跃龙
作者单位:1. 100052,北京,中国疾病预防控制中心病毒病预防控制所国家流感中心,传染病预防控制国家重点实验室
2. 中国疾病预防控制中心应急办
摘    要:目的 阐明2004-2005年中国流行的B型流感病毒血凝素抗原性及其基因变异情况.方法 对2004-2005年分离的B型毒株先进行单向血凝抑制试验;在此基础上选取不同时间、地点的B型流感毒株进行血凝素基因HA1区核苷酸序列测定并推导出其氨基酸序列,然后进行基因进化特性分析.结果 2004-2005年我国人群中同时流行着B型Yamagata系和Victoria系毒株.Yamagata系毒株与B/Shanghai/361/02比较,2004年有3.7%病毒单向血凝抑制效价有4倍以上差异,2005年有4.5%病毒单向血凝抑制效价有4倍以上差异,并且在血凝素基因HA1区发生9个氨基酸替换,在196为增加一个糖基化位点.Victoria系毒株与B/Hong kong/330/01比较,2004年有8.5%病毒单向血凝抑制效价有4倍以上差异,2005年有20.6%病毒单向血凝抑制效价有4倍以上差异,并且在HA1区发生9个氨基酸替换,在197位增加一个糖基化位点.结论 2004-2005年我国人群中流行的B型流感病毒的抗原性与B/Shanghai/361/02、B/Hong kong/330/01相比抗原性已经发生了变化.

关 键 词:流感病毒B型  抗原特性  血凝素类
收稿时间:2006-03-13
修稿时间:2006年3月13日

Antigenic and genetic study of influenza virus B circulated in China in 2004-2005
ZHANG Ye,WEN Le-ying,ZHAO Xiang,LI Zi,GUO Jun-feng,XU Cui-ling,WANG Min,YU Hong-jie,YANG Wei-zhong,GUO Yuan-ji,SHU Yue-long.Antigenic and genetic study of influenza virus B circulated in China in 2004-2005[J].Chinese Journal of Experimental and Clinical Virology,2006,20(2):11-13.
Authors:ZHANG Ye  WEN Le-ying  ZHAO Xiang  LI Zi  GUO Jun-feng  XU Cui-ling  WANG Min  YU Hong-jie  YANG Wei-zhong  GUO Yuan-ji  SHU Yue-long
Institution:Chinese Influenza Center, Institute for Viral Disease Control and Prevention, State Key Laboratory for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Abstract:BACKGROUND: To analyze the genetic and antigenic characteristics of hemagglutinin (HA) gene of human influenza B virus isolated from the mainland of China since 2004-2005. METHODS: The single-way hemagglutinin inhibition (HI) tests were used to test the antigenic characteristics, and the HA1 gene was sequenced based on the antigenic results. RESULTS: The Yamagata-like and Victoria-like viruses co-circulated in 2004-2005. For the Yamagata-like virus, the single-way HI results showed that 3.7% and 4.5% of the viruses had 4-fold greater HI titer difference compared with B/Shanghai/361/02 in 2004 and 2005, respectively. The HA1 sequence data showed that the virus had amino acid mutation, and there was one more glycosylation site at 196th site. For the Victoria-like virus, the single-way HI results showed that 8.5% and 20.6% of the viruses had 4-fold greater HI titer difference compared with B/Hong kong/330/01 in 2004 and 2005, respectively. The HA1 sequence data showed that the virus had replacement of 9 amino acids, and there was one more glycoaylation site at 197th site. CONCLUSION: The results showed that influenza B viruses had changed antigenic and genetic characteristics compared with B/Shanghai/361/02, B/Hong kong/330/01 in 2004-2005.
Keywords:Influenza B virus  Antigenic characteristics  Hemagglutinins
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