乙肝母婴阻断"成功"儿童外周血中检出HBV DNA

目的 检测乙型肝炎母婴阻断成功儿童血清HBV DNA,提出应该完善母婴阻断效果评价指标的建议.方法 采集乙肝疫苗母婴阻断后HBsAg阴性的儿童血清标本,经试剂盒提取HBVDNA,采用巢式PCR方法 扩增HBV DNA S区基因片段后纯化测序.结果 140例儿童血标本中,12例HBV DNA阳性,阳性率8.6%;S基因测序检测未发现突变株.结论 鉴于血清学检测方法 敏感性低于PCR扩增方法 .在评价乙型肝炎母婴阻断效果时,加入HBV DNA指标,可使其更完善.

Detection of HBV DNA among HBsAg negative children after prevention for mother-to-infant transmission

Objective Detection HBV DNA among HBsAg negative children who have been vaccined at birth, in order to improve the evaluation of the indicator for HBV DNA infection. Methods Selection HBsAg negative children who have been vaccined at birth and then detection HBV DNA from sera using QIAamp Viral DNA Mini Kit, HBV DNA s region was obtained by nested PCR and sequencing. Results 12 of the 140 chidren were HBV DNA detected were positive and the infectious rate was 8.6%. No mutant of the 12 HBVDNA in "a" determinant. Conclusion To evaluate the effection of the prevention of HBV mother-to-child transmission, the standard method should be established. The detection of HBV DNA should be included in the future.