血清和肝组织TGF-β1与慢性乙型肝炎肝纤维化的关系

目的 探讨血清和肝组织TGF-β1水平与慢性乙肝肝纤维化程度的关系,为肝纤维化诊断提供依据.方法 以肝活检病理诊断区分131例慢性HBV感染者纤维化程度(S0～S4),用ELISA法检测血清TGF-β1水平,免疫组化法检测肝组织TGF-β1表达并半定量.分析血清TGF-β1和肝组织TGF-β1表达与肝纤维化程度的关系.结果 血清和肝组织TGF-β1均与肝纤维化程度具有非常显著性正相关(r分别是0.74和0.89,P＜0.01).血清TGF-β1各组间比较差异有统计学意义(P＜0.01).组问分割比较,S0和S1分别与S4比较差异均有统计学意义(P＜0.005);各组与S0组比较差异均有统计学意义(P＜0.005);S1组和S3组之间比较有统计学意义(P＜0.005).肝组织TGF-β1表达在S3和S4组之间比较差异无统计学意义(P＞0.05),其余组间比较差异均有统计学意义(P＜0.01).血清TGF-β1和肝组织TGF-β1表达具有非常显著性意义相关(r=0.61,P＜0.01).结论 血清TGF-β1和肝组织TGF-β1水平与慢乙肝肝纤维化程度相关,血清TGF-β1有希望成为临床判断轻度或重度肝纤维化的无创伤性诊断指标.

Level of serum and liver tissue TGF-β1 in patients with liver fibrosis due to chronic hepatitis B

Objective To detect the level of Serum and liver tissue TGF-β1 in patients with chronic hepatitis B, to study their relation to liver fibrosis and gain the evidence for diagnosis of liver fibrosis. Methods The liver fibrosis grades(S0-S4) of 131 cases with chronic HBV infection were diagnosed after liver biopsy. Serum TGF-β1 was detected by enzyme-linked immunosorbent assay, and the semiquantitative analysis was applied after detecting the expression of TGF-β1 in liver tissue with immunohistochemistry. Their relations to liver fibrosis were analyzed. Results Serum and tissue level of TGF-β1 increased significantly with the development of fibrosis, and the same result was obtained between themselves(P＜0.01). There was very significant difference for Serum level of TGF-β1 among the groups with different fibrosis grades (P＜0.01). Serum levels of TGF-β1 were decreased significantly comparing the Group S0 or S1 to S4(P＜0.005). There were significant difference for Serum level of TGF-β1 among S0 and the others(P＜0.005). And there was significant difference between S1 and S3(P＜0.005) .The expression level of TGF-β1 in liver tissue has no significant difference between group S3 and S4 (P＞0.05). However, the differences were significantly among the others comparisons(P＜0.01). Conclusion There is close relation between the level of TGF-β1 and the different liver fibrosis grades due to chronic hepatitis B. The serum level of TGF-β1 is a potential noninvasive maker for diagnosis of liver fibrosis.