人疱疹病毒6型感染与口腔鳞癌关系的初步研究

目的研究人疱疹病毒6型(HHV-6)感染与口腔鳞癌的关系。方法用间接免疫荧光试验及聚合酶链反应,分别检测口腔鳞癌和对照组患者血清中抗HHV-6 IgG及外周血单个核细胞中HHV-6 DNA序列;用免疫组化染色法检测口腔鳞癌组织标本中HHV-6抗原。结果16例口腔鳞癌患者和16例其他口腔疾病患者血清中抗HHV-6 IgG的阳性数分别为16例和12例,几何平均滴度分别为1:118和1:64,差异有显著意义(P<0.05)。上述两组患者HHV-6 DNA的检出数分别为10例和2例(P<0.05)。其中12例口腔鳞癌组织标本经免疫组化染色检测,9例(9/12)HHV-6抗原阳性,而8例对应癌旁组织中,阳性者仅有2例(2/8),两者间差异有显著意义(P<0.05)。结论HHV-6可能是口腔鳞癌发生的诱因,在肿瘤发生发展中起一定作用。

Relationship between human herpesvirus 6 infection and oral squamous cell carcinoma

Objective To study the relationship between human herpesvirus 6 (HHV-6) and oral squamous cell carcinoma. Methods The serum anti-HHV-6 antibody titers from oral squamous cell carcinoma patients and control subjects were detected by indirect immunofluorescence assay. HHV-6 DNA in peripheral blood mononuclear cells from oral squamous cell carcinoma patients and control subjects was amplified by PCR with primers from sequence of HHV-6 and the specificity was confirmed by Southern-blot hybridization with an internal probe oligonuclotide. An immunohistochemical staining using rabbit anti-HHV-6 antibody was used to detect HHV-6 antigen in oral tumor tissues from oral squamors cell carcinoma patients. Results Significantly higher proportion of patients with oral carcinoma (16/16) had IgG antibody to HHV-6 in sera compared with those (12/16) in control subjects, and geometric mean titer of these two groups was 1:118 and 1:64 respectively ( P < 0.05) . The detectable rate of HHV-6 DNA in peripheral blood mononuclear cells for the above groups was 10/16 and 6/16 respectively ( P < 0.05). HHV-6 antigens were positive in 9 out of 12 oral tumor cases and in only 2 out of 8 pericancerous tissues, the difference between these two groups was also significant ( P < 0.05) . Conclusion These results demonstrated the frequent presence of HHV-6 in oral squamous cell carcinoma, therefore, HHV-6 possibly play a role in the pathogenesis of oral squamous cell carcinoma.