| 常见呼吸道病毒分子鉴别诊断技术的建立 |
| |
| 引用本文： | 邹淑梅,韩健,温乐英,Kassi Cronin,舒跃龙.常见呼吸道病毒分子鉴别诊断技术的建立[J].中华实验和临床病毒学杂志,2006,20(2):17-20. |
| |
| 作者姓名： | 邹淑梅 韩健 温乐英 Kassi Cronin 舒跃龙 |
| |
| 作者单位： | 1. 100052,北京,中国疾病预防控制中心病毒病所,传染病预防控制国家重点实验室
2. Genac Biomedical Products,INC |
| |
| 基金项目： | 国家自然科学基金资助（30599433） |
| |
| 摘 要： | 目的 建立一种常见呼吸道感染病毒的快速检测方法,为尽早诊断、减少疾病的传播以及为临床提供良好的治疗依据.方法 采用液体芯片检测技术结合靶向多重RT-PCR技术建立可以同时检测13种呼吸道病原的检测技术.结果 该方法特异性方面检测13种常见呼吸道病毒没有交叉,标本的检出率为100%;灵敏度方面达到10e2-10e1（pfu/ml）.结论 该分子鉴别诊断可应用常见呼吸道病毒的检测,协助诊断病毒引起的病毒性呼吸道感染.
|
| 关 键 词： | 分子鉴别诊断 呼吸道病毒 |
| 收稿时间： | 2006-03-13 |
| 修稿时间： | 2006年3月13日 |
Establishment of molecular differential diagnostic (MDD) technology for detection of common respiratory viruses |
| |
| ZOU Shu-mei,HAN jian,WEN Le-ying,Kassi Cronin,SHU Yue-long.Establishment of molecular differential diagnostic (MDD) technology for detection of common respiratory viruses[J].Chinese Journal of Experimental and Clinical Virology,2006,20(2):17-20. |
| |
| Authors: | ZOU Shu-mei HAN jian WEN Le-ying Kassi Cronin SHU Yue-long |
| |
| Institution: | State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China |
| |
| Abstract: | Objective To provide rapid laboratory evidence for diagnosis of respiratory infection and help diagnose accurately and reduce the spread of disease, so that the patients can be diagnosed and treated early. Methods Thirteen kinds of respiratory viruses were detected by using the Genaco's MDD technology. Results All the specimens were detected, the total positive rate was 100%; the sensitivity of the method was 10e2(pfu/ml). Conclusion The MDD system can distinguish the 13 respiratory viruses, which helps diagnosis of respiratory viral infection. |
| |
| Keywords: | Molecular differential diagnostic Respiratory virus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
