烟碱型乙酰胆碱受体α3和/或α5型在大鼠脑垂体中的表达:荧光双标免疫组织化学研究

为了探讨在大鼠脑垂体中,包含α3和 /或α5亚单位的烟碱型乙酰胆碱受体(N -AChR)是否存在,我们运用免疫荧光双标记技术对成年雄性大鼠脑垂体进行了研究。结果显示:N -AChR的α3和 /或α5亚单位样免疫反应阳性物质局限性地分布在神经垂体的催产素能神经纤维上,并一直延续到神经垂体的尾侧末端。

THE EXPRESSION OF α3 AND/OR α5 SUBUNITS OF NICOTINIC ACETYLCHOLINE RECEPTOR IN RAT PITUITARY: DOUBLE IMMUNOFLUORESCENCE MICROSCOPY STUDY

To explore the existence of the α3 and/or α5 subunits of nicotinic acetylcholine receptor (N-AChR) in rat pituitary, we used the double immunofluorescence staining method on the adult male rat pituitary tissue. We found that α3 and/or α5 subunits of N-AChR-like immunoreactivity (-LI) was exclusively shown on the oxytoinergic fibers of neurohypophysis, and this kind of fibers was seen to be extended to the caudal end of the neurohypophysis.