磷酸化的细胞外信号调节蛋白激酶1/2在正常小鼠脑内的免疫细胞化学定位

细胞外信号调节蛋白激酶(ERK)1/2是重要的信号转导分子。现已知在正常动物的中枢神经系统内有其活化形式即磷酸化的ERK1/2(pERK1/2)分子的存在,但其在小鼠脑内的分布目前还没有全面的观察。本研究用免疫组织化学技术(ABC法)研究了pERK1/2样免疫反应阳性产物在脱臼处死的正常小鼠全脑内的分布。结果发现pERK1/2在正常小鼠脑内有广泛的表达,阳性产物主要存在于神经元,亦见于个别白质内的胶质细胞,脑膜及室管膜细胞也有表达。强阳性表达的核团主要有:岛皮质、视听皮质、边缘前皮质、扣带前皮质、海马垂直部、弓状核、蓝斑和小脑Purkinje细胞;中等阳性表达的核团主要有:感觉运动皮质、外侧隔区、内侧杏仁核、皮质杏仁核和外侧杏仁核、丘脑室旁核前部、视交叉上核、穹隆下器、终板血管器、前腹侧视前核和下丘脑背内侧核;弱阳性表达的核团主要有:视上核、下丘脑室旁核大细胞部、下丘脑后区、顶盖前区、室周灰质腹外侧柱、A5区、孤束核和延髓腹外侧网状结构等。本文结果观察到pERK1/2在正常小鼠脑内广泛存在,提示pERK1/2作为重要的信号转导分子,可能参与许多脑区在正常状态下的功能活动,揭示其分布特点为了解其在脑内的多样性功能提供了形态学依据。

THE IMMUNOCYTOCHEMICAL LOCALIZATION OF PHOSPHORY- LATED EXTRACELLULAR SIGNAL-REGULATED KINASES 1/2 IN THE MOUSE BRAIN

Extracellular signal-regulated kinases (ERK)1/2 is a pivotal molecule for signaling transduction. It is known that the phosphorylated ERK1/2 (pERK1/2), the activated form of ERK1/2, is widely distributed in the normal mammalian brains, but the comprehensive study on its location in the mouse brain has not been performed. In this study immunocytochemistry technique (ABC method) was used to observe the distribution of pERK1/2-like immunoreactive cells in the mouse brain following cervical dislocation. The results showed a widespread expression of pERK1/2 in the neurons in discrete areas, some pERK1/2 immunoreactivities were also observed in glia as well as in the meningeal and ependymal cells in the mouse brain. The insular, visual, auditory, prelimbic and anterior cingulated cortices, perpendicular portion of hippocampus, arcuate nucleus, locus coeruleus and Purkinje cells in cerebellum were strongly stained; the sensory and motor cortices, lateral septum, medial, cortical and lateral subnuclei of amygdala, anterior part of thalamic paraventricular nucleus, suprachiasmatic nucleus, and dorsomedial nucleus of hypothalamus, and anteroventral preoptic nucleus were moderately stained; while the supraoptic nucleus, magnocellular part of hypothalamic paraventricular nucleus, posterior hypothalamic area, pretectal region, ventrolateral column of periaqueductal gray of the midbrain, A5 cell group, nucleus of solitary tract, rostroventrolateral and caudoventrolateral medulla oblongata were only faintly stained. The results from present study indicate that pERK1/2, as a well-known key molecule in signal transduction, might be involved in regulating functional activities in many brain regions in normal animal, and the current data has provided morphological basis for further confirmation of this hypothesis.