| 类风湿关节炎小鼠关节聚集的T细胞克隆型变化的分析 |
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| 引用本文： | 赵文明,山本一彦.类风湿关节炎小鼠关节聚集的T细胞克隆型变化的分析[J].细胞与分子免疫学杂志,2004,20(1):70-72. |
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| 作者姓名： | 赵文明 山本一彦 |
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| 作者单位： | 1. 首都医科大学免疫学系,北京,100054
2. 东京大学医学部变态反应和类风湿科,日本 |
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| 摘 要： | 目的 :通过对类风湿性关节炎 (RA)小鼠模型发病不同时期的四肢足关节聚集的T细胞克隆型的分析 ,了解T细胞克隆型与RA病变的关系。方法 :用RT PCR/SSCP法 ,对一种自发性RA小鼠模型 (SKG小鼠 )发病初期与后期四肢足关节聚集的T细胞克隆型进行比较分析。结果 :在发病后期 ,四肢足关节中一致的Vβ2和Vβ8.2T细胞克隆型均明显升高 (分别为 72 .3%和 6 0 .2 % )。结论 :TCR为Vβ2和Vβ8.2的T细胞克隆 ,可能对于SKG小鼠RA的发展起重要作用
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| 关 键 词： | T细胞克隆型 类风湿关节炎 RT-PCR SSCP |
| 文章编号： | 1007-8738(2004)01-0070-03 |
| 修稿时间： | 2003年1月21日 |
Analysis of the change in clonotypes of T cells accumulated in 4 feet joints of SKG mice |
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| ZHAO Wen-ming ,YAMAMOTO Kazuhiko.Analysis of the change in clonotypes of T cells accumulated in 4 feet joints of SKG mice[J].Journal of Cellular and Molecular Immunology,2004,20(1):70-72. |
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| Authors: | ZHAO Wen-ming YAMAMOTO Kazuhiko |
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| Institution: | Department of Immunology, Capital University of Medical Sciences, Beijing 100054, China. zhao-wenming@163.com |
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| Abstract: | AIM: To understand the relationship between T cell clonotypes and RA invasion through clonotypes of analysis of T cells accumulated in 4 feet joints of SKG mice. METHODS: Comparative analysis of T cells clonotypes of accumulated in the joints of SKG mice, a spontaneous RA mouse model, was carried out by RT-PCR/SSCP at the initial and late-stages of RA. RESULTS: The identical rate of Vbeta2 and Vbeta8.2 T cell clonotypes in 4 feet joints increased significantly at the late stage of RA (72.3% and 60.2%, respectively). CONCLUSION: The T cells with Vbeta2 or Vbeta8.2 TCR may play an important role in the RA development of SKG mice. |
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| Keywords: | T cell clonotype rheumatoid arthritis RT-PCR SSCP |
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