蝎毒重组蛋白对人肺腺癌A549细胞的抑制作用及其机制

目的 探讨蝎毒重组蛋白对人肺腺癌A549细胞的抑制作用及其抗肿瘤机制.方法 四甲基偶氮唑蓝(MTT)比色法检测蝎毒重组蛋白对人肺癌A549细胞增殖的抑制作用;荧光显微镜及流式细胞仪(FCM)技术观测蝎毒重组蛋白对肿瘤细胞内钙离子浓度(Ca2+]I)的影响,探讨Ca2+]I变化时Ca2+的可能来源.结果 蝎毒重组蛋白对人肺癌A549细胞生长有明显抑制作用;荧光显微镜显示,蝎毒重组蛋白作用细胞48 h后细胞内钙离子荧光强度增强,提示Ca2+]I升高;流式细胞仪显示蝎毒重组蛋白在不同情况下可显著增加肿瘤Ca2+]I(P<0.05).结论 蝎毒重组蛋白对人肺腺癌A549细胞增殖具有显著抑制作用,可能与升高肿瘤Ca2+]I继而诱导肿瘤细胞凋亡有关;其升高肿瘤Ca2+]I是通过开放肿瘤细胞膜钙通道和肿瘤细胞内钙库释放两条途径实现.

Inhibitory effect and mechanism of recombinant protein of buthus martensii venom on human lung adenocarcinoma cell line A549

Objective To investigate the inhibitory effect and antitumor mechanism of recombinant protein of buthus martensii venom on A549 human lung adenocarcinoma cell line.Methods The inhibitionof proliferation in vitro was measured by methyl thiazolyl tetrazolium (MTT)assay.Influence of Ca2+]i by recombinant protein of buthus martensii venom was observed by fluorescence microscope and flow cytometry.And then to explore the source of Ca2+ whenCa2+]i was aherating.Results The protein could inhibit A549cell proliferation.Fluorescent microscope assay showed enhanced fluorescent intensity after 48 hours,and itmeaned the increasingCa2+]i concentration.Flow cytometry assays showed the recombinant protein can significantly increase the concentration ofca2+]i in tumor cells under different circumstances(P<0.05).Conclusion The recombinant protein can obviously inhibit the proliferation of A549 cell line,and itsmechanism has relationship with tumor cell apoptosis through increasingCa+]i level of tumor cell asresulted by openning the calcium ion channel on the membrane and releasing calcium ion in tumor cells.