Taxol对宫颈癌U14细胞增殖、凋亡及α2,6-SA和ST6Gal mRNA表达的影响

目的:研究泰素(Taxol)对小鼠宫颈癌U14细胞株增殖、凋亡以及α2,6-唾液酸(SA)和α2,6-唾液酸转移酶(ST6Gal)mRNA表达的影响,为进一步探讨宫颈癌Taxol化疗机制提供新思路。方法:Taxol处理U14细胞后,MTT检测Taxol对U14细胞的IC_(50)值。流式细胞术检测细胞α2,6-SA、细胞凋亡相关因子(Bcl-2、Bax、caspase8和caspase 3)、凋亡率和细胞周期改变。qPCR检测ST6Gal1和ST6Gal2 mRNA的表达。结果:与对照组相比,Taxol对U14细胞有明显的抑制作用,减弱α2,6-SA荧光强度,上调Bax表达,下调Bcl-2表达,降低Bcl-2/Bax比值,并增强caspase 8和caspase 3活性;Taxol处理显著增加U14细胞凋亡率及S期细胞和G2/M期细胞比率,此外还下调ST6Gal1 mRNA表达。结论:α2,6-SA和ST6Gal可能参与了Taxol对U14细胞细胞周期和凋亡调控的多重作用。

Effects of Taxol on proliferation,apoptosis, and mRNA expression of α2,6-sialic acid and ST6Gal in cervical carcinoma cell line U14

AIM: To study the effect of Taxol on the proliferation, apoptosis, and mRNA expressions of α2,6-sialic acid (SA) and α2,6-sialyltransferase (ST6Gal) in mouse cervical cancer cell line U14.METHODS: After the U14 cells were treated with Taxol, the IC₅₀ value of Taxol to U14 cells was detected by MTT assay. The expression of α2,6-SA and apoptosis-related factors (Bcl-2, Bax, caspase 8 and caspase 3), the apoptosis rate and cell cycle were determined by flow cytometry. The mRNA expression of ST6Gal1 and ST6Gal2 was detected by qPCR.RESULTS: As compared with control group, Taxol induced obvious U14 cell growth inhibition, reduced α2,6-SA expression, up-regulated Bax, down-regulated Bcl-2, decreased the ratio of Bcl-2/Bax, enhanced caspase 8 and caspase 3 activity, increased the apoptotic rate and cell proportions of Sub-G₁ and S phases, and induced G₂/M phase arrest. Taxol also down-regulated the mRNA expression of ST6Gal1, and slightly up-regulated the mRNA expression of ST6Gal2.CONCLUSION: α2,6-SA and ST6Gal are involved in the multiple effects of Taxol on modulation of the cell cycle and apoptosis in U14 cells.