基质金属蛋白酶3和尿激酶型纤溶酶原激活物受体在2型糖尿病大血管病变中的变化

目的： 探讨基质金属蛋白酶3（MMP-3）和尿激酶型纤溶酶原激活物受体（uPAR）在2型糖尿病大血管病变中的变化。方法: 用ELISA双抗体夹心法测定26例正常对照和39例2型糖尿病患者（其中单纯2型糖尿病15例、合并大血管病变24例）的血浆MMP-3和uPAR水平。结果: 单纯糖尿病组uPAR高于正常对照（P<0.05）而MMP-3无显著差异；合并大血管病变组MMP-3显著高于正常对照和单纯糖尿病组（P<0.01，P<0.01），而uPAR亦高于正常对照及单纯病变组（P<0.01，P<0.05）。2型糖尿病血浆MMP-3水平和uPAR水平呈正相关， LDL-C与uPAR呈正相关且是uPAR主要影响因素。 结论: MMP-3和uPAR与2型糖尿病大血管病变发生密切相关，MMP-3可作为2型糖尿病血管病变的循环标志物。

Changes of matrix metalloproteinase-3 and urokinase-type plasminogen activator receptor in type 2 diabetes mellitus with macroangiopathy

AIM: To investigate the function of matrix metalloproteinase-3 (MMP-3) and urokinase-type plasminogen activator receptor (uPAR) to macroangiopathy in type 2 diabetes mellitus. METHODS: The levels of MMP-3 and uPAR in plasma were determined by ELISA sandwich method in 26 healthy controls and 39 patients with type 2 diabetes mellitus including 15 complication-free cases and 24 with macroangiopathy. RESULTS: The plasma level of uPAR but not MMP-3 was higher in patients without macroangiopathy than that in normal controls (P<0.05). In patients with macroangiopathy, the plasma levels of MMP-3 and uPAR were higher than those in normal controls (P<0.01) and patients without angiopathy. There was positive relationship between MMP-3 and uPAR in type 2 diabetes mellitus (r=0.405, P<0.05), and LDL-C was the important affecting factor of uPAR. CONCLUSION: MMP-3 and uPAR are associated with the development of macroangiopathy in type 2 diabetes mellitus. MMP-3 can be regarded as the circulatory markers in type 2 diabetes mellitus with macroangiopathy.