线粒体相关内质网膜在顺铂诱导卵巢癌细胞凋亡中的作用

目的:探讨卵巢癌细胞SKOV3在顺铂的作用下,对线粒体相关内质网膜(MAMs)的影响。方法:用6 mg/L顺铂处理SKOV3细胞0、12和24 h;通过间接免疫荧光法检测顺铂对active caspase-3的蛋白表达水平以及B细胞受体相关蛋白31(BAP31)和电压依赖性阴离子通道蛋白1(VDAC1)的共定位的影响;流式细胞术检测细胞凋亡率的变化;并通过透射电镜观察线粒体相关内质网膜的变化。结果:激光共聚焦结果显示,顺铂诱导卵巢癌SKOV3细胞中active caspase-3蛋白水平升高,同时也增加了BAP31和VDAC1的共定位;流式细胞术结果表明,顺铂可明显诱导细胞凋亡;透射电镜结果表明顺铂增加了线粒体和内质网之间的关联(P0.05)。结论:顺铂诱导了SKOV3细胞凋亡,并伴有线粒体和内质网之间关联的增加,推测线粒体相关内质网膜可能参与了顺铂诱导卵巢癌细胞凋亡的过程。

Mitochondria associated endoplasmic reticulum membranes in cisplatin induced ovarian cancer cell apoptosis

AIM: To explore the structural change of mitochondria associated endoplasmic reticulum membranes (MAMs) in SKOV3 cells exposed to cisplatin. METHODS: The SKOV3 cells were treated with cisplatin at concentration of 6 mg/L. The protein levels of active caspase-3, as well as the colocalization of B-cell receptor-associated protein 31 (BAP31) and voltage-dependent anion channel protein 1 (VDAC1) in the SKOV3 cells were determined by the method of indirect immunofluorescence. The apoptotic rate of the SKOV3 cells was analyzed by flow cytometry. The structural change of MAMs was observed under transmission electron microscope. RESULTS: Under the confocal microscope, we found that cisplatin increased the protein levels of active caspase-3 as well as colocalization of BAP31 and VDAC1 in the SKOV3 cells. The results of flow cytometry demonstrated that cisplatin increased the apoptotic rate of the SKOV3 cells (P<0.05). The results of transmission electron microscopy showed that cisplatin induced increase in mitochondrial-associated membrane structures (P<0.05). CONCLUSION: Cisplatin induces SKOV3 cell apoptosis with increased MAMs contacts. MAMs may play a role in cisplatin induced SKOV3 cell apoptosis.