MMP-2 基因启动子区-735C→T多态性与冠状动脉粥样硬化的相关性研究

目的: 研究中国汉族人群基质金属蛋白酶2( MMP-2 )基因启动子区-735C→T多态性与冠状动脉粥样硬化(CAS)的相关性。方法: 用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法,以309例经冠状动脉造影确诊为冠状动脉粥样硬化的患者为研究对象,检测 MMP-2 基因启动子区-735C→T多态性,以311例同期体检的正常人为对照组,比较两组间 MMP-2 基因多态性频率分布的差异,并结合冠脉造影情况,探讨 MMP-2 基因多态性与CAS发病及冠状动脉狭窄程度的关系。结果: CAS组CC基因型频率(86.1%)明显高于对照组(79.7%),而CT+TT基因型频率(13.9%)明显低于对照组(20.3%),两组之间的差异显著(P<0.05); CAS组C等位基因频率(92.6%)高于对照组(89.1%),T等位基因频率(7.4%)低于对照组(10.9%),两组差异显著(P<0.05)。 MMP-2 基因-735C→T多态性与冠状动脉狭窄程度无关(P>0.05)。结论: MMP-2 基因-735C→T多态性可能与中国汉族人群CAS有关, MMP-2 基因CC基因型和C等位基因可能是CAS遗传易感性的基因标记之一。

Relationship between matrix metalloproteinase 2-735C→T genetic polymorphism in promoter region and coronary atherosclerosis

AIM: To investigate the relationship between matrix metalloproteinase 2 ( MMP-2 )-735C→T polymorphism in the promoter region and coronary atherosclerosis (CAS) in Han population of China. METHODS: This study was conducted with a CAS group including 309 patients confirmed by angiography and 311 control healthy subjects. Genotype of -735C→T functional promoter polymorphism of the MMP-2 gene was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The relationship between the polymorphism in MMP-2 gene and CAS was analyzed. RESULTS: The frequency of CC genotype (86.1%) in CAS group was significantly higher than that in control group (79.7%), but the frequency of CT+TT genotype (13.9%) in CAS group was significantly lower than that in control group (20.3%). The statistical difference between CAS group and controls was significant(χ²=4.398,P<0.05). The frequency of -735C in CAS group (92.6%) was higher than that in control group (89.1%) and the frequency of -735T in CAS group (7.4%) was lower than that in control group (10.9%), with the statistical significant difference (χ²=4.521, P<0.05). The degree of stenosis in coronary artery did not significantly relate to the MMP-2 gene -735C→T polymorphism in the promoter region. CONCLUSION: The genetic polymorphism in MMP-2 promoter region (-735C→T) is associated with the susceptibility to CAS in Han population of China. CC genotype and C allele may be a genetic marker. The -735C→T polymorphism may be useful as a predictor of CAS.