胰腺癌P53蛋白表达与AgNORs量及组织学分级的关系

目的：研究胰腺癌P53蛋白表达与癌细胞核仁组成区嗜银蛋白（AgNORs）量及组织学分级的关系。方法：用免疫组织化学法检测56例胰腺癌组织及25例正常胰腺组织中突变型P53蛋白的表达； AgNORs染色技术检测胰腺癌细胞的AgNORs含量；观察肿瘤的组织学分级。结果： 56例胰腺癌中P53蛋白表达阳性28例（50％），正常胰腺细胞均为阴性，P53蛋白在胰腺癌组织和正常胰腺组织中的表达有显著差异(P<0.01)。28例P53蛋白阳性的胰腺癌组织，AgNORs计数为9.14±2.08；28例P53蛋白阴性的胰腺癌中，AgNORs计数为5.99±1.84，二者有显著差异（P<0.01）。P53蛋白表达与肿瘤组织学分级呈显著负相关。不同的组织学分级之间，AgNORs计数有显著差异（P<0.01）。结论：胰腺癌发生发展的过程包含了〖STBX〗p53〖STBZ〗基因的突变。P53蛋白表达和AgNORs含量可反映胰腺癌的恶性程度，是一个有效的判断胰腺癌预后的指标。

The relationship between expression of p53 protein and AgNORs count and histological grade in pancreatic adenocarcinoma

AIM: To study the relationship between expression of p53 protein and AgNORs count and histological grade in pancreatic adenocarcinoma．METHODS: Fifty-six patients with pancreatic adenocarcinoma and 25 normal pancreatic tissue were examined for mutant P53 protein by immunohistochemistry, silver binding nucleolar organizer regions（AgNORs）were counted in cancer cells, and histological grade was observed in pancreatic adenocarcinoma. RESULTS: P53 protein positive expression was 50% in pancreatic adenocarcinoma, P53 protein expression was negative in all normal pancreatic tissue. There is a significant difference in P53 expression between normal pancreatic tissue and pancreatic adenocarcinoma tissue. The mean AgNORs count was 9.14±2.08 in group with P53 protein positive expression, and 5.99±1.84 in group with P53 protein negative expression group. The P53 expression was associated with AgNORs significantly. The P53 expression was significantly related with histological grade, and AgNORs count was also significantly related with histological grade. CONCLUSION: P53 may be closely related to carcinogenesis and development of pancreatic adenocarcinoma. P53 protein expression and AgNORs count might be a useful prognostic factor in pancreatic adenocarcinoma.