虎杖配伍山楂抗动脉粥样硬化易损斑块病变的机制研究

目的:观察解毒中药虎杖配伍活血中药山楂对载脂蛋白E基因敲除小鼠ApoE(-/-)小鼠]病理形态的影响和炎症因子的变化,以探讨中药虎杖配伍山楂改善动脉粥样硬化(AS)易损斑块病变的可能调控机制。方法:将高脂饲养的ApoE(-/-)小鼠分为虎杖组、山楂组、虎杖加山楂配伍组、血脂康组、高脂饲料模型组,并设ApoE(-/-)小鼠普通饲料对照组和C57BL/6J小鼠普通饲料对照组,共分7组,每组12只小鼠。连续灌胃给药17周,取血检测炎症因子超敏C反应蛋白(hs-CRP);取主动脉,光镜观察组织结构;免疫组织化学法检测核因子-κB(NF-κB)蛋白表达。结果:高脂饲养各组小鼠及普通饲料模型组小鼠主动脉均可见不同程度的AS病理改变。各治疗组给予虎杖、山楂、虎杖加山楂配伍、血脂康后均能减轻小鼠主动脉病变情况。高脂饲料模型组血清NF-κB和hs-CRP水平均显著高于正常对照组和普通饲料组;各给药组均能明显降低NF-κB和hs-CRP表达水平,与高脂模型组相比差异显著(P0.01),其中以虎杖加山楂配伍组降低最明显。结论:虎杖加山楂配伍可能通过降低NF-κB和hs-CRP表达,发挥抗AS的形成、发展和稳定易损斑块的作用。

Effect of polygonum cuspidatum and hawthorn on anti-atherosclerosis unstable plaque

AIM:To investigate the effect of detoxifying herbs polygonum cuspidatum,and hawthorn,herb of promoting blood flow,on pathologic morphology and inflammatory factors in apolipoprotein E gene knockout mice,in order to approach the possible regulatory mechanism of polygonum cuspidatum and hawthorn for treating artherosclerosis (AS) unstable plaque. METHODS: The animals were divided into 7 groups (12 mice in every group). The ApoE (-/-) mice fed with high fat diet were divided into polygonum cuspidatum group,haw...