Apocynin降低1型糖尿病小鼠心肌组织炎症因子的表达

目的： 探讨NADPH氧化酶抑制剂apocynin对1型糖尿病小鼠心肌组织IL-1β、IL-18、TNF-α和COX-2表达的影响及其机制研究。方法: C57小鼠腹腔注射STZ建立1型糖尿病动物模型，44只8周龄雄性小鼠分为对照组（sham，n=6）、apocynin对照组（Apo，n=6）、1型糖尿病组（STZ，n=16）及1型糖尿病apocynin治疗组(STZ+Apo,n=16)。实验于8周末结束，运用超声心动图检测小鼠心脏功能，以实时定量RT-PCR技术测定心肌组织IL-1β、IL-18、TNF-α和COX-2 mRNA表达，以Western blotting检测心肌组织磷酸化Akt表达。结果: 1型糖尿病小鼠心脏功能降低伴随心肌组织IL-1β、IL-18、TNF-α和COX-2 mRNA表达增加，STZ+Apo组小鼠心脏功能增高伴随心肌组织IL-1β、IL-18、TNF-α和COX-2 mRNA表达减少；1型糖尿病小鼠心肌组织磷酸化Akt显著减少，STZ+Apo组心肌组织磷酸化Akt明显增加。结论: Apocynin提高1型糖尿病小鼠心脏功能伴随显著减少心肌组织IL-1β、IL-18、TNF-α和COX-2 mRNA的表达，其机制可能与增加心肌组织磷酸化Akt表达密切相关。

Apocynin decreases cardiac cytokines in STZ-induced diabetic cardiomyopathy

AIM: This study was to investigate the effects of apocynin on the expression of IL - 1β, IL - 18, TNF -α and COX -2 mRNA in the diabetic caidiomyopathy heart. METHODS: Diabetes was induced in 8 - week old C57 mice with one intraperitoneal injection of streptozotocin (STZ, 100 mg/kg). The experiments were divided into 4 groups; sham (n =6) , apocynin control (Apo, n=6) , diabetic cardiomyopathy (STZ, n = 16) and apocynin treatment (STZ + Apo, n= 16). At the end of 8 weeks after induction of diabetes, cardiac functions were evaluated by echocardio-graphy. Cardiac tissue was analyzed for the expression of IL - 1β, IL - 18, TNF - α, COX - 2 mRNA and phosphorylated Akt by real - time RT - PCR and Western blotting, respectively. RESULTS: The expression of IL - 1β, IL -18, TNF - α and COX - 2 mRNA increased in diabetic cardiomyopathy heart accompanied with cardiac dysfunction. Cardiac functions were improved with decreased levels of IL - 1β, IL - 18, TNF - α and COX - 2 mRNA expression in apocynin - treated heart. Compared with reduction of phosphorylated Akt, apocynin significantly increased phosphorylation of Akt in diabetic cardiomyopathy heart. CONCLUSION: Apocynin decreases cardiac IL - 1β, IL - 18, TNF - α and COX - 2 mRNA expression accompanied with improved cardiac function, which associates with the increase of phosphorylated Akt in diabetic cardiomyopathy heart.