E-cadherin表达缺失在白血病细胞行为中的作用

目的:确定上皮-钙黏蛋白(E-cadherin)表达缺失在白血病细胞行为中的作用，最终阐明E-cadherin介导的骨髓微环境与造血细胞相互作用改变在白血病发生中的作用。 方法:细胞黏附实验测定 HL-60细胞经5-氮杂脱氧胞苷（5-Aza-CdR）诱导E-cadherin表达后细胞黏附能力的改变，MTT法测定细胞增殖能力，细胞迁移实验确定细胞的迁移能力。结果:HL-60细胞经5-Aza-CdR诱导E-cadherin表达后，其对E-cadherin-Fc的黏附能力增加，细胞在E-cadherin-Fc上的增殖能力下降，细胞黏附导致细胞迁移能力下降。结论:白血病细胞中由于E-cadherin表达丧失，引起细胞黏附降低，增殖能力增强，细胞迁移活跃，表明E-cadherin表达缺失与白血病细胞行为的获得有关。

Influence of absent expression of E-cadherin on leukemic cell behaviors

AIM:To investigate the influence of absent expression of E-cadherin on leukemic cell behavior and further determine the role of abnormal interactions between hematopoietic progenitor and bone marrow microenvironment on leukogenesis.METHODS:After E-cadherin was induced with 5-Aza-CdR in HL-60 leukemic cells, cell adhesion ability was determined by cell adhesion assay.E-cadherin mediated adhesion associated proliferation was examined by MTT assay, cell mobility was determined by a transwell migration assay.RESULTS:After E-cadherin was induced by 5-Aza-CdR in HL-60 cells, the adhesion ability of cells with E-cadherin-Fc was enhanced.E-cadherin mediated adhesion then inhibited HL-60 cells proliferation and suppressed cell migration ability.CONCLUSION:Absent expression of E-cadherin on leukemic cells plays a role in the decreased adhesion, enhanced proliferation and increased migration ability of leukemic cells.Loss of E-cadherin expression is responsible for the behaviors of leukemia cells.