三七总皂苷通过Notch3/Hes-1/p27Kip1信号通路抑制低氧性肺动脉高压大鼠肺血管重构

目的：探讨Notch3/Hes-1/p27^Kip1信号通路与低氧性肺动脉高压大鼠肺血管重构的关系及三七总皂苷(PNS)的干预作用。方法：将SPF级雄性SD大鼠采用随机编号分为3组：正常组(C组)，低氧组(H组)和PNS组(P组)，检测肺动脉压，称量右心室重量，HE染色和Masson染色观察肺血管重构情况，RT-qPCR检测大鼠肺组织中Notch3、Hes-1和p27^Kip1的mRNA表达水平，Western blot检测大鼠肺组织中Notch3、Hes-1、p27^Kip1，增殖细胞核抗原(PCNA)和caspase-3的蛋白水平。结果：与C组相比，H组大鼠的肺动脉压和右心室肥厚指数明显增加；肺血管重构现象明显；Notch3、Hes-1和PCNA的蛋白水平增加，p27^Kip1和caspase-3的蛋白水平降低；Notch3和Hes-1的mRNA表达增加，p27^Kip1的mRNA表达降低(P<0.05)。经PNS干预后，与H组相比，P组大鼠的肺动脉压和右心室肥厚指数降低；肺血...

Panax notoginseng saponins inhibit pulmonary vascular remodeling in hypoxic pulmonary hypertensive rats via Notch3/Hes-1/p27;signaling pathway

AIM:To explore the effects of Panax notoginseng saponin(PNS)on hypoxia pulmonary hypertension(HPH)-induced pulmonary vascular remodeling in rats.METHODS:Male SD rats were randomly divided into 3 groups:normoxic control group(C group),hypoxia group(H group)and PNS group(P group). Mean pulmonary artery pressure,right ventricular hypertrophy index and pulmonary vascular remodeling were detected. The protein levels of Notch3,Hes1,p27^Kipl,proliferating cell nuclear antigen(PCNA)and caspase-3 were measured by Western blot. The mRNA expression levels of Notch3,Hes-1 and p27;were measured by RT-qPCR.RESULTS:Pulmonary hypertension,right rentricular hypertrophy and pulmonary vascular remodeling were observed in HPH model rats,with increased expression of Notch3,Hes-1 and PCNA,and decreased expression of p27;and caspase-3(P<0. 05). Furthermore,PNS treatment alleviated pulmonary hypertension and right rentricular hypertrophy,and inhibited hypoxia-induced pulmonary vascular remodeling. Treatment with PNS also inhibited the expresion of Notch3,Hes-1 and PCNA,and stimulated the expresion of p27;and caspase-3(P<0. 05).CONCLUSION:Treatment with PNS attenuates HPH pulmonary vascular remodeling in rats by inhibition of Notch3/Hes-1/p27;signaling pathway.