粒细胞集落刺激因子动员对供者外周血细胞树突状细胞亚群的影响

目的：研究粒细胞集落刺激因子(G-CSF)动员对外周血单个核细胞（PBMNC）中树突状细胞（DC）亚群及其功能的影响。方法：以CD34^-Lin^- HLA-DR⁺细胞群设门4色荧光分析方法检测25例无关供者G-CSF动员前、后外周血细胞DC亚群；ELISA检测其血清相关细胞因子IL-12p40、IL-10、IFN-γ、IL-4活性，并分析DC₁/DC₂（CD11c⁺CD123^- /CD11c^-CD123⁺）比值与CD34⁺细胞含量的相关性。结果：G-CSF动员后, 供者PBMNC 的DC₁/DC₂比值显著低于动员前（P<0.05）；DC₁/DC₂与CD34⁺细胞含量呈负相关（r=-0.438, P<0.05），CD34⁺细胞/MNC≥0.4%时， DC₁/DC₂倒置；而血清相关细胞因子IL-12p40、IL-10、IFN-γ、IL-4水平无显著改变（P>0.05）； DC₂ HLA-DR表达上调而CD83不表达。结论： G-CSF动员后，供者外周血CD34⁺细胞数量增加的同时，DC₂数量也增加，这些DC₂尽管HLA-DR表达上调，但仍处前体细胞状态，不直接分泌或调节Th2细胞分泌免疫抑制因子。

Effect of G - CSF on dendritic cell subsets in donor's mobilized peripheral blood cells

AIM: To explore the effect of granulocyte colony stimulating factor (G-CSF) on donor’s dendritic cell （DC）subsets in peripheral blood cells (PBC). METHODS: The subsets of dendritic cells in PBC were analyzed by CD34^-Lin^- HLA-DR⁺ cells and the levels of IL-12p40, IL-10, IFN-γ, IL-4 in the serum were tested by ELISA before (25 cases) or after G-CSF mobiling. The relationship between the ratio of DC₁/DC₂ (CD11c⁺CD123^-/CD11c^-CD123⁺) and CD34⁺/MNC was explored. RESULTS: CD34⁺/MNC in PBC harvests was above 0.4% in 23 cases, and the ratio of DC₁/DC₂ was lower, the HLA-DR expression of DC₂ was enhanced after G-CSF mobiling than before, but the levels of IL-12p40, IL-10, IFN-γ, IL-4 in donors serum and CD83 expression of DC₂ were not changed by G-CSF. CONCLUSION: DC₂ increased accompanied by the increase in CD34⁺ cells in the PBC harvests. Although the expression of HLA-DR in DC₂ was up-regulated by G-CSF, these DC₂ did not regulate Th2 cells to excrete inhibitor cell factors and kept on the precursor characters.