Nestin在CDA-2诱导人胶质瘤细胞分化中的变化

目的： 研究nestin（巢蛋白）在人尿萃取物细胞分化剂CDA-2（又名尿多酸肽）诱导SWO-38人胶质瘤细胞分化中表达的变化及其意义。方法: 采用光镜观察和鉴定CDA-2对SWO-38细胞的分化作用；RT-PCR、细胞免疫荧光、Western blotting分析CDA-2诱导前后SWO-38细胞nestin表达的变化。 结果: 光镜观察发现经CDA-2诱导后SWO-38细胞胞体变大、核浆比减小、突起增多，表现出向成熟的星形细胞分化的现象；Nestin表达在细胞浆，呈丝状着色；Nestin在CDA-2诱导SWO-38细胞分化中表达下调。结论: Nestin在CDA-2诱导SWO-38细胞分化中表达下调，进一步验证nestin与细胞分化的关系，nestin有可能成为胶质瘤诱导分化治疗领域新的标志物。

Change of nestin expression in differentiation of human glioma cells induced by CDA-2

AIM: To investigate the change and significance of nestin expression in differentiation of human glioma cell line SWO-38 induced by CDA-2 (uroacitide, a healthy human urine extract). METHODS: Cellular differentiation of SWO-38 cells induced by CDA-2 was determined by light microscopy. The change of nestin expression in SWO-38 cells induced by CDA-2 was detected by munofluorescence, RT-PCR and Western blotting. RESULTS: Light microscopic observation revealed that CDA-2 induced SWO-38 cells to differentiate into astrocytes with increased cytoplasm and cytoplasmic processes and decreased nucleus/cytoplasm ratio. Nestin was expressed in cytoplasm and stained like filament by immunofluorescence staining. Nestin expression was downregulated in differentiated SWO-38 cells induced by CDA-2. CONCLUSION: Nestin expression is downregulated in differentiation of SWO-38 cells induced by CDA-2, which verifies the relationship between nestin expression and cell differentiation. Nestin may be a new differentiation marker of glioma.