EGCG通过下调COX-2和MMP-2表达抑制人脑胶质瘤细胞的迁移和侵袭

目的:探讨表没食子儿茶素没食子酸酯(EGCG)下调COX-2及MMP-2的表达、抑制人脑胶质瘤细胞迁移及侵袭的作用机制。方法:MTT法检测EGCG处理24h后SWO-38细胞的活性,确定药物作用浓度;细胞侵袭与迁移实验检测EGCG处理24h后SWO-38细胞的迁移与侵袭能力;Western blotting对比分析EGCG处理24h后SWO-38细胞COX-2和MMP-2的表达。通过肿瘤坏死因子α(TNF-α)促进COX-2的表达,观察EGCG是否通过COX-2/MMP-2通路抑制肿瘤的迁移侵袭。结果:细胞侵袭与迁移实验发现,EGCG对SWO-38细胞的迁移和侵袭能力有抑制作用,与对照组SWO-38细胞相比较,有显著差异(P0.01)。Western blotting发现经过EGCG处理24h后,SWO-38细胞COX-2和MMP-2蛋白的表达水平降低,提示EGCG抑制SWO-38迁移的机制可能与该药物抑制COX-2的表达而降低SWO-38细胞酶解细胞外基质的能力相关。结论:EGCG抑制了人脑胶质瘤SWO-38细胞的迁移与侵袭,其机制与EGCG抑制COX-2表达后,调节了MMP-2诱导的酶解细胞外基质的能力相关。

EGCG depresses migration and invasion of human glioma cell line by downregulating COX-2 and MMP-2 expression

AIM: To investigate the mechanism that epigallocatechin-3-gallate (EGCG) depresses the migration and invasion in human glioma cell line SWO-38 by downregulation of cyclocxygenase-2(COX-2) and matrix metalloproteinase-2(MMP-2). METHODS: The effect of EGCG on the apoptosis of SWO-38 cell line was examined by the method of MTT. The migration and invasion of the SWO-38 cells were determined by Transwell assay. The expression of COX-2 and MMP-2 was measured by Western blotting. Meanwhile, TNF-α was used to stimulate the expression of COX-2 for determining if the mechanism of COX-2 pathway is involved in the inhibitory effect of EGCG on the migration and invasion of the tumor cells. RESULTS: After treated with EGCG for 24 h, the migration and invasion abilities of SWO-38 cells were lower than that of the cells before treatment. The results of Western blotting revealed that the 24 h treatment of EGCG on SWO-38 cell line inhibited the expression levels of COX-2 and MMP-2, indicating that the degradation of the extracellular matrix in SWO-38 cells was related to the COX-2 signaling pathway. CONCLUSION: EGCG inhibits the migration and invasion of SWO-38 cell line. The correlation between COX-2 expression and enzymatic degradation in the extracellular matrix determines the abilities of migration and invasion of tumor cells.