软脉降脂合剂干预动脉搭桥术内皮细胞损伤的研究

 目的：观察软脉降脂合剂对兔动脉搭桥术后内皮细胞功能的影响，探讨软脉降脂合剂在动脉搭桥术后再狭窄发生过程中的防治作用，以及中药治疗冠状动脉搭桥术（CABG）后再狭窄的可能作用机制。方法：新西兰大白兔随机分为4组：模型组、阿司匹林对照组、软脉降脂中剂量组和软脉降脂高剂量组，行右侧颈动脉搭桥术。术后给药灌胃8周后，取抗凝血用流式细胞仪检测循环内皮细胞（CECs），取血清用ELISA检测P-选择素和细胞间黏附分子1(ICAM-1)水平。结果：各给药组和模型组比较，CECs相对比例、P-选择素和ICAM-1明显降低，差异有统计学意义（P<0.05）。软脉降脂合剂2组与阿司匹林对照组比较差异也有统计学意义（P<0.05）。结论：中药软脉降脂合剂能够降低动脉搭桥术后的CECs、P-选择素和ICAM-1水平，有助于改善CABG术后内皮细胞的功能。

Effect of Ruanmaijiangzhi mixture intervention on endothelial cell function after artery bypass graft

AIM： To observe the effect of Ruanmaijiangzhi mixture on the function of endothelial cells in rabbits after artery bypass graft. METHODS：A New Zealand white rabbit model of right common carotid artery bypass graft was established. The rabbits were randomly divided into 4 groups： model group, aspirin control group, middle dose of Ruanmaijiangzhi group and high dose of Ruanmaijiangzhi group. Eight weeks after treatment, circulating endothelial cells (CECs) in anticoagulated blood were detected by flow cytometry. The serum levels of P-selectin and intercellular adhesion molecule 1 (ICAM-1) were measured by ELISA. RESULTS：Compared with model group, the relative ratio of CECs, and the levels of P-selectin and ICAM-1 in medication administration groups were significantly decreased. The effect in Ruanmaijiangzhi groups was better than that in aspirin control group. Ruanmaijiangzhi treatment showed a positive dose-related correlation. CONCLUSION：Chinese medicine Ruanmaijiangzhi mixture decreases CECs, P-selectin and ICAM-1 to improve the endothelial cell function after coronary artery bypass graft.