| LIHGT-HVEM/LTbR途径缺陷诱导T细胞的失能 |
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| 引用本文： | 尚宇航,姜曼,杨菲,曹朝晖,董世访,江伟凡,陈戬,吴玉章,许桂莲.LIHGT-HVEM/LTbR途径缺陷诱导T细胞的失能[J].免疫学杂志,2010(9). |
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| 作者姓名： | 尚宇航 姜曼 杨菲 曹朝晖 董世访 江伟凡 陈戬 吴玉章 许桂莲 |
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| 作者单位： | 第三军医大学基础部免疫学教研室;南华大学生物化学与分子生物学教研室; |
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| 基金项目： | 国家自然科学基金(30971394); 教育部归国人员启动基金(2009); 重庆市科委自然科学基金(2008BB5021); 第三军医大学回国人员启动基金(2007XG43) |
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| 摘 要： | 目的探讨LIGHT-HVEM/LTbR信号通路缺陷对T细胞应答的影响。方法以LIGHTKO小鼠为动物模型,制备脾脏单细胞悬液,加入抗CD3mAb和抗CD28mAb刺激。通过3H-TdR掺入法检测了细胞的增殖能力、ELISA和FACS分别检测了细胞培养上清和胞内IFN-gamma和IL-10的水平,并探讨了这些细胞对外源性rIL-2的反应性。结果与野生型的C57BL/6小鼠相比,LIGHTKO小鼠的脾细胞表现为低下的增殖活性(P<0.05);细胞培养上清中Th1细胞因子IFN-gamma的水平显著下降(P<0.05);与ELISA结果一致,FACS胞内染色结果表明,IFN-gamma阳性T细胞的百分率显著下降,而LIGHTKO小鼠脾细胞IL-10的产生却显著高于野生型的对照小鼠(P<0.01)。进一步的研究表明LIGHTKO小鼠这种缺陷的IFN-gamma产生主要与CD8+T细胞亚群有关,而CD4+T细胞亚群IFN-gamma的产生是正常的。外源性rIL-2的加入可使LIGHTKO小鼠T细胞恢复正常的增殖能力和IFN-gamma的产生。结论 LIGHT-HVEM/LTbR信号通路缺陷导致T细胞的失能。
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| 关 键 词： | LIGHT-HVEM/LTbR 失能 T细胞 |
Induction of T cells anergy by the deficiency of LIGHT-HVEM/LTbR signal pathway |
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| SHANG Yuhang,JIANG Man,YANG Fei,CAO Zhaohui,DONG Shifang,JIANG Weifan,CHEN Jian,WU Yuzhang,XU Guilian.Induction of T cells anergy by the deficiency of LIGHT-HVEM/LTbR signal pathway[J].Immunological Journal,2010(9). |
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| Authors: | SHANG Yuhang JIANG Man YANG Fei CAO Zhaohui DONG Shifang JIANG Weifan CHEN Jian WU Yuzhang XU Guilian |
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| Institution: | SHANG Yuhang,JIANG Man,YANG Fei,CAO Zhaohui,DONG Shifang,JIANG Weifan,CHEN Jian,WU Yuzhang,XU Guilian Department of Immunology,Third Military Medical University,Chongqing 400038,China |
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| Abstract: | To explore the effects of LIGHT-HVEM/LTbR signal pathway deficiency on the T cells response,LIGHT KO mice was used as an animal model in this study. Single cell suspension from the spleen was prepared and stimulated with purified anti-CD3 mAb plus anti-CD28 mAb in vitro. Then cell proliferation was determined by 3H-TdR incorporation,while the cultural supernatant and intracellular IFN-gamma and IL-10 levels were measured by ELISA and FACS,respectively. In contrast to WT C57BL/6 mice,cells from LIGHT KO mice... |
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| Keywords: | LIGHT-HVEM/LTbR Anergy T cell |
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