| 用FPLC系统提纯可溶型Ⅱ型胶原蛋白 |
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| 引用本文： | 钱娴娟,胡永秀,赵文明.用FPLC系统提纯可溶型Ⅱ型胶原蛋白[J].免疫学杂志,2001,17(3):228-230. |
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| 作者姓名： | 钱娴娟 胡永秀 赵文明 |
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| 作者单位： | 首都医科大学免疫学系, |
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| 摘 要： | 目的 采用FPLC系统提取纯化可溶型Ⅱ型胶原蛋白（CⅡ）。方法 采用鸡剑突软骨。经胃蛋白酶消化离心，再使用FPLC系统进行DEAESepharoseFF高效液相镦子交换层析和脱盐柱脱盐，得到纯化的CⅡ。结果 自提的CⅡ纯品与CⅡ标准品电泳结果带型一致。结论 本法提纯蛋白效率高。重复性好，快速简便。
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| 关 键 词： | 可溶型Ⅱ型胶原蛋白 FPLC系统 提纯 |
| 文章编号： | 1000-8861（2001）03-0228-03 |
| 修稿时间： | 2000年12月11 |
Isolation and purification
of soluble type |
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| QIAN Xian-juan,HU Yong-xiu,ZHAO Wen-ming WTBX.Isolation and purification
of soluble type[J].Immunological Journal,2001,17(3):228-230. |
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| Authors: | QIAN Xian-juan HU Yong-xiu ZHAO Wen-ming [WTBX] |
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| Institution: | QIAN Xian-juan,HU Yong-xiu,ZHAO Wen-ming [WT5BX] |
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| Abstract: | Objective To isolate and purify soluble type Ⅱcollagen by FPLCsystem． Methods Xiphoid cartilage of chickens was digested with pepsin and was centrifuged to obtain the rough protein solution． Then， FPLC system was adopted to carry on DEAE ion exchange chromatography and desalting chromatography in order to purify the protein． Results It was found that the bands of CⅡsamples and standard CⅡwere at the same location by SDS-PAGE． Conclusions The improved method we adopted had significant advantages such as the high purification efficiency，excellent stability and the fast and simple working process compared with traditional method． |
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