葡萄球菌肠毒素超抗原广谱抑制性多肽的功能研究

目的在前期筛选出针对SEA、SEB、SEC具有广谱抑制性的多肽P72基础上,通过竞争结合实验和动物模型对多肽P72的抑制机制进行探讨。方法采用竞争结合实验检测多肽P72与MHCⅡ类分子的亲合力;利用"两次攻击(two-hit)法"建立的动物模型研究P72对SEs的体内抑制活性。结果 P72不能与FITC-SEs有效竞争结合Raji细胞上的MHCⅡ类分子,P72对SEA、SEB和SEC致Balb/c小鼠休克效应具有显著的保护作用。结论 P72可能不是与MHC II类分子结合而产生的抑制作用,P72能够在体内抑制SEs的超抗原活性,其具体的抑制机制有待深入研究。

Study on the function of a broad-spectrum inhibitory peptide against SEs superantigen

This study aims to investigate the mechanism of the broad-spectrum inhibitory activity of synthetic peptide P72 against SEA,SEB and SEC based on the previous research of competition assay and animal experiments.We detected the binding ability of the peptide P72 to MHC class Ⅱ molecules by competition assay and assessed the in vivo biological activity of peptide P72 against SEs by the "two-hit" animal model.The results indicated that the peptide P72 could not bind to MHC class Ⅱ molecules,while P72 can completely protect most of the Balb/c mice against toxic shock induced by SEA,SEB and SEC.In conclusion,our study demonstrates that the inhibitory activity of peptide P72 may not due to binding to MHCⅡ.And peptide P72 can inhibit the biological activity of SEs in vivo.But the exact mechanism of inhibitory activity of P72 still needs studies.