Abp1在调节性T细胞的发育及功能中的作用

目的探讨肌动蛋白连接蛋白1(actin-binding protein 1,Abp1)对调节性T细胞(Treg)发育及功能的影响。方法通过流式细胞术检测野生型小鼠(WT组)和Abp1基因敲除小鼠(KO组)的胸腺、脾脏、浅表淋巴结及肠系膜淋巴结中T淋巴细胞及Treg细胞的比例、数量以及相关功能性分子的表达,并利用体外抑制试验检测Abp1缺失对Treg抑制功能的影响。结果与WT小鼠相比,Abp1 KO小鼠的胸腺、脾脏、浅表淋巴结及肠系膜淋巴结中的Treg的比例和细胞数目均没有明显的变化,同时缺失Abp1的Treg细胞的主要功能性分子ICOS、CTLA4、PD-1和NRP-1的表达亦没有显著的差异,进一步实验发现缺失Abp1的Treg细胞对初始T细胞向活化T细胞分化的抑制作用较WT相比无明显变化。结论Abp1对Treg细胞的发育和功能没有显著影响。

Roles of Abp1 in the development and function of regulatory T cells

This study was designed to investigate the effect of progranulin actin-binding protein 1(Abp1)on the differentiation and function of regulatory T cell,and the molecule mechanism.We detected the proportion,number and functional molecule expression of T lymphocytes and Treg cells in the thymus,spleen,peripheral lymph nodes and mesenteric lymph nodes of wild-type mice(WT group)and Abp1 knockout mice(KO group)by cytometry flow,and the effect of Abp1 deletion on the inhibitory function of Treg in vitro was measured as well.Compared with WT mice,Abp1 KO mice had no significant differences on the proportion and number of Tregs in the thymus,spleen,peripheral lymph nodes and mesenteric lymph nodes,meanwhile,the expression of the main functional molecules ICOS,CTLA4,PD-1 and NRP-1 in Treg cells lacking Abp1 were not significantly different.Furthermore,Abp1-absent Treg cells showed no significant changes in inhibiting T cells activation as comparing with WT Treg cells.In conclusion,Abp1 has no significant effect on the development and function of Treg cells.