乳腺癌个体中差异表达lncRNA的研究

目的 识别乳腺癌个体中差异表达长链非编码RNA (long non-coding RNA,lncRNA).方法 应用LncRIndiv方法识别乳腺癌个体中差异表达lncRNA,并使用超几何检验进行亚型分析和Log-rank检验进行预后分析.结果 lncRNA差异判断的平均准确率高于96％.分别识别出1 81个、32个、15个和72个basal-like、HER2-enriched、luminal A、luminal B 亚型特异的lncRNAs(False discovery rate＜0.05,超几何检验).LncRNA ZNF582-ASl差异下调的乳腺癌患者生存差(TCGA,P=0.038;GSE42568,P=0.026,Log-rank检验),是潜在的预后标志.结论 LncRIndiv方法识别个体中差异表达lncRNA准确性高.乳腺癌个体差异表达lncRNA可用来识别亚型特异的lncRNA和预后标志.

Individualized investigations of differentially expressed lncRNAs in breast cancer patients

Objective To identify differential expression of long non-coding RNA (lncRNAs) in breast cancer patients individually.Methods LncRIndiv was used to detect differentially expressed lncRNAs in individual breast cancer patients.Hypergeometric test and log-rank test were used to identify subtype-specific lncRNAs and prognosis-related lncRNAs.Results The average precision for LncRIndiv was more than 96％.Totally,181,32,15 and 72 lncRNAs were defined as basal-like,HER2-enriched,luminal A-and B-specific lncRNAs,respectively (False discovery rate ＜ 0.05,hypergeometric test).Breast cancer with down-regulation of ZNF582-AS1 showed poorer prognosis than patients without down-regulation of ZNF582-AS1 (TCGA,P =0.038;GSE42568,P =0.026,Log-rank test),which is a potential prognostic biomarker.Conclusion LncRIndiv show high precision in detection of individually differential expression of lncRNAs.The differentially expressed lncRNAs in individual breast cancer can be applied to detect subtype-specific lncRNAs and prognostic lncRNA.