Anti-tumor effect and mechanism of cyclooxygenase-2 inhibitor through matrix metalloproteinase 14 pathway in PANC-1 cells |
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| Authors: | Siyuan Li Zhuoyu Gu Zhiwei Xiao Ting Zhou Jun Li Kan Sun |
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| Institution: | 1.Medical College of Shihezi University, Key Laboratory of Ministry of Education, Xinjiang Endemic and Ethnic Diseases, Shihezi 832002, China;2.Department of Endocrinology, First Affiliated Hospital, Medical College of Shihezi University, Shihezi 832002, China;3.Key Laboratory, First Affiliated Hospital, Medical College of Shihezi University, Shihezi 832002, China |
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| Abstract: | Objective: To investigate whether celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, can attenuate proliferation, migration, invasion and MMP-14 expression in pancreatic cancer cells PANC-1 and the possible anti-tumor mechanism of celecoxib. Methods: Human pancreatic cancer cell line PANC-1 cells were treated with diverse concentrations of celecoxib (20, 60, 100 μmol/L). Cell proliferation, invasion and migration capabilities were measured by MTT colorimetry, transwell invasion assay, and scratch assay separately. At the same time, the protein expression of COX-2 and MMP-14 was assessed by ELISA. Results: The capabilities of proliferation, invasion and migration in PANC-1 cells were attenuated in a concentration-dependent manner after treated with celecoxib, followed by the down-regulation of the protein expression of COX-2 and MMP-14. In addition, MMP-14 expression was significantly positively correlated with COX-2 expression. Conclusions: COX-2 inhibitor celecoxib can inhibit the proliferation, invasion and migration of PANC-1 cells via down-regulating the expression of MMP-14 in a concentration-dependent manner, thus contributing to its anti-tumor effect in pancreatic cancer. |
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| Keywords: | Pancreatic cancer celecoxib matrix metalloproteinase-14 cyclooxygenase-2 |
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