Plasticity of Human Mesenchymal Stem Cell Phenotype and Expression Profile under Neurogenic Conditions |
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Authors: | A V Shakhbazov N V Goncharova S M Kosmacheva N A Kartel’ and M P Potapnev |
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Institution: | 1.Institute of Cytology and Genetics,National Academy of Sciences of Belarus,Minsk,Belarus;2.Center of Hematology and Transfusiology,Minsk,Belarus |
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Abstract: | Human bone marrow MSC cultured in neurogenic medium containing EGF and FGFb demonstrated alteration of the phenotype and expression
of neuronal precursor/early neuron markers nestin and NSE. Signals of expression of neuronal and oligodendroglial markers
MAP-2, dm-20, and MBP were detected after prolongation of incubation in neurogenic medium to 2 weeks. Cells with neuronal
morphology were immunopositive to early neuronal marker β-III-tubulin. Replacement of neurogenic medium for α-MEM with 10%
fetal calf serum induced reversion of the phenotype to that typical for human MSC. This indicates high plasticity of the phenotype
and expression profile of neuronal markers in MSC cultured under neurogenic conditions or possibility of dedifferentiation
of MSC reaching the stage of neuronal precursors/early neurons. |
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