家兔坐骨神经损伤后肌肉组织乙酰胆碱酯酶的变化

目的 :探讨坐骨神经损伤后不同时间点家兔小腿肌肉组织乙酰胆碱酯酶 (AChE)的变化。方法 :建立家兔坐骨神经损伤的动物模型 ,制备特异识别神经系统AChE的单克隆抗体 ,以该抗体作为一抗 ,酶标羊抗兔IgG作为二抗建立酶联免疫检测方法 ;测定正常对照组、坐骨神经神经损伤后 0 .5h、3h、12h组 ,坐骨神经断点、胫神经及腓总神经所支配的肌肉组织AChE的含量。结果 :坐骨神经损伤 0 .5h后 ,断点处AChE含量最高 ,波长 492nm之OD值为 ( 0 .64 0± 0 .0 68) ,随时间延长逐渐降低 ;胫神经、腓总神经支配的肌肉组织中AChE含量 0 .5h时最低 ,波长 492nm之OD值分别为 ( 0 .2 13± 0 .0 72 )、( 0 .2 0 3±0 .0 60 ) ,3h最高 ,12h时降低。结论 :坐骨神经损伤后 ,断点及其远端神经支配的肌肉组织中AChE的变化呈时间依赖性 ,但变化不一。

The change of acetylcholinesterase concentration in muscle after ischiadicus nerve injury in rabbit

Objective: To study the change of acetylcholinesterase(AChE) concentration in muscle tissues after its dominating nerves were injured at different time point.Methods:The rabbit sciatic nerve injury model was established,and the monoclonal antibody against nervous AchE was prepared.Using this antibody as the first antibody and selecting antibody IgG HRP from sheep to rabbit as second antibody,the ELISA method was found.By these methods,the AChE concentrations of muscle in different groups were detected.Results:The AChE concentration of nerve arranging muscle were the highest after sciatic nerve injury at 0.5 hour time point,then it descended gradually.The AChE concentration in the muscle dominated by tibial nerve and common peroneal nerve were the lowest at 0.5 hour time point after being injured and the change of them were the highest at 3 hour point.All of them were declining at 12 hour point.Conclusion:The changes of AChE concentration in different muscle were different,and it depend on the time point after nerve injured.