活性氧调控因子1在人退变椎间盘中的表达及意义

目的　探讨活性氧调控因子1（reactive oxygen species modulator 1，ROMO1）在退变椎间盘髓核中的表达。 方法　采用免疫组化和荧光探针DCFH-DA检测不同退变髓核组织中ROMO1的表达与(reactive oxygen species, ROS)含量，并分析ROMO1表达水平与ROS含量、椎间盘退变程度的相关性。 结果　各组ROMO1的平均光密度值依次为0.192±0.089、0.328±0.048、0.399±0.053、0.468±0.098；各组ROS结果分别为132.961±15.149、191.889±17.880、218.056±12.845、243.501±30.279。随着椎间盘退变程度的加重， ROMO1蛋白表达量与ROS含量逐渐升高，且差异具有显著性(P<0.05)。ROMO1的表达水平与ROS的含量及椎间盘退变程度均呈正相关关系（P＜0.05）。 结论　ROMO1的表达水平与椎间盘的退变程度呈正相关，这可能与其增加髓核组织内ROS含量而加重氧化应激损伤有关。

The expression and significance of active oxygen modulation factor 1 in human intervertebral disc degeneration

Objective　To explore the expression and their relationship of ROMO1 and ROS in the degenerative intervertebral disc.　Methods　Immunohistochemical techniques and fluorescent probes DCFH-DA were applied to detect the expression of ROMO1 and ROS levels in the different degenerated nucleus pulposus.　Results　The mean option density of ROMO1 were 0.192±0.089, 0.328±0.048, 0.399±0.053, and 0.468±0.098, respectively. The results of ROS detection were 132.961±15.149, 191.889±17.880, 218.056±12.845, and 243.501±30.279， separately，in the groups of A, B, C, and D. It showed the expression of ROMO1 and the content of ROS increased gradually with the aggravation of intervertebral disc degeneration, and there was a positive correlation between ROMO1 expression level and the content of ROS in intervertebral disc (r=0.732, P<0.05). There was also a positive correlation between ROMO1 expression level and the intervertebral disc degeneration degree (r=0.603, P<0.05).　Conclusion　ROMO1 and ROS are highly expressed in the nucleus pulposus tissue of the degenerated intervertebral disc, which has a positive correlation; and the expression level of ROMO1 is positively correlated with the degree of degeneration of intervertebral disc, which might be related to aggravated oxidative stress injury by increasing the content of ROS in the nucleus pulposus.