| DNA微阵列的数字图像处理 |
| |
| 引用本文： | 郝立巍,华如梅,李树祥,韩慧霞.DNA微阵列的数字图像处理[J].中国医学物理学杂志,2002,19(1):17-19. |
| |
| 作者姓名： | 郝立巍 华如梅 李树祥 韩慧霞 |
| |
| 作者单位： | 1. 全军重点医学图像研究室
2. 全军重点肿瘤研究所,广东广州,510515 |
| |
| 摘 要： | 从微阵列图像中提取基因表达和识别基因，并进一步测定基因的功能是DNA微阵列研究的主要目标，而微阵列图像处理通过定位和提取每个cDNA靶点上探针的信号强度，并通过计算平均灰度值和分析强度比率等为生物学家进行更高层的基因分析提供必要条件，本文介绍采用红绿双色荧光标记杂交实验进行微阵列图像处理几个关键步骤；微阵列靶区的分割，背景强度的提取，靶点检测，靶点灰度提取、靶点表达程度分析。
|
| 关 键 词： | 图像处理 DNA微阵列 基因表达 |
| 文章编号： | 1005-202X(2002)01-0017-03 |
| 修稿时间： | 2001年6月12日 |
Digital image processing of DNA microarray |
| |
| HAO Li-wei ,HUA Ru-mei ,LI Shu-xiang ,HAN Hui-xia.Digital image processing of DNA microarray[J].Chinese Journal of Medical Physics,2002,19(1):17-19. |
| |
| Authors: | HAO Li-wei HUA Ru-mei LI Shu-xiang HAN Hui-xia |
| |
| Institution: | HAO Li-wei 1,HUA Ru-mei 1,LI Shu-xiang 1,HAN Hui-xia 2 |
| |
| Abstract: | The main objective of the microarray research work is to measure,the expressions of the genes and recognize them so that the function of every specific gene can be found or tested.The practical approach through image processing is to locate every DNA microarray target and extract probe intensities on the target.By measuring their mean value of the target pixels and calculating the ratio(R/G)biologists can perform further high-level analysis.This paper introduces several key steps of microarray image analysis based on the hybridization experiment of two fluorescent probes tagged with different colors (R and G).The steps are array target segmentation,background intensity extraction,target detection,target intensity extraction,ratio distribution analysis. |
| |
| Keywords: | image processing DNA microarray gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
