终板蛋白多糖的变化对颈椎间盘力学性能的影响

目的:通过测定退变颈椎间盘压缩性能的改变,同时观测软骨终板基质蛋白多糖(proteoglycan PG)的变化,为退变的椎间盘显现异常力学特性作一分子机制上的探讨。方法:将24只家兔随机分为对照组和模型组,模型组家兔保持45°低屈曲住5小时/次·天,取C5-6椎间盘进行生物力学测定;同时生化测定椎间盘终板糖胺多糖(glycosaminoglycan,GAG)总量、硫酸软骨素(chongdroitin sulphate,CS)和硫酸角质素(keratan sulphate,KS)比值和透明质酸(hyaluronic acid,HA)含量;总结分析颈椎间盘退变时基质各成分的系统变化及其对椎间盘力学性能的影响。结果:模型组终板抗压强度、断裂时的最大形变、终板基质蛋白多糖总含量、硫酸软骨素/硫酸角质素比值、透明质酸含量都低于对照组(P<0.05),并随造模进程的深入而减小。结论:长时间的应力不均状态,造成椎间盘终板材料力学特性改变;颈椎间盘基质蛋白多糖含量和成分改变是颈椎间盘生物力学性能减退的主要原因之一。

The effect of the proteoglycan content of cartilage endplate on biomechanics behavior of the cervical intervertebral disc

Objective: To probe the molecular base for abnormal biomechanics behavior on the cervical intervertebral disc. Methods: Twenty-four Japanese white rabbits were randomly divided into control and model group. The model rabbits were kept al 45?flexion lor 5 hours once daily. C5-6 disc was chosen to determine the biomechanical characters, the GAG content,ratio of choridroitin sulphate(CS) to keratan sulphate(KS) and hyaluronic acid(HA) content. The relations between biomechanical behavior changes and the proteoglycan content were summed up. Results: The model group showed significant decrease of antipress intension and largest shape change,GAG content ,CS/KS ratio,HA content. Conclusion: The material mechanics changes of interver-bral disc can be induced by long time abnormal stress; The changes of proteoglycan content may be the major reason of inter-verbral disc degeneration.