| 急性白血病患者外周血HMGA1基因的表达 |
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| 引用本文： | 郝明巨,李稳,刘敏,叶翎.急性白血病患者外周血HMGA1基因的表达[J].微循环学杂志,2009,19(4):38-40. |
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| 作者姓名： | 郝明巨 李稳 刘敏 叶翎 |
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| 作者单位： | [1]山东省中医院检验科,济南250011 [2]济南市皮肤病医院检验科,济南250011 [3]济宁市第一人民医院血液科,济南250011 |
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| 摘 要： | 目的:检测急性白血病(AL)患者外周血高迁移率蛋白A1(HMGA1)基因表达水平。方法:采用SYBR Green I RQ-PCR检测30例AL,包括25例AML(M2～M7)和5例ALL(AL组),25例非白血病患者(对照组)外周血HMGA1 mRNA表达水平。结果:AL组HMGA1 mRNA表达明显高于对照组,ALL和AML间HMGA1 mRNA表达水平无显著差异;AML中M7的HMGA1 mRNA表达水平高于M2～M5。结论:HMGA1 mRNA在AL患者外周血高表达,其中M7水平最高,可作为白血病基因治疗的新靶点及M7的鉴别诊断指标。
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| 关 键 词： | 高迁移率蛋白A1基因 SYBR Green I实时定量聚合酶链反应急性白血病 外周血 |
Detection of HMGA1 Expression in Peripheral Blood of Acute Leukemia Patients with SYBR Green I Real-time Quantitative RT-PCR |
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| Abstract: | Objective:To investigate high-mobility group A1 (HMGA1)mRNA expression levels in peripheral blood(PB) of acute leukemia patients (ALs).Method: To adopt SYBR Green I real-time quantitative polymerase chain reaction(RQ-PCR) for detecting HMGA1 in PB of 30 ALs including 25 AMLs(M2～M7)and 5 ALLs,25 non-leukemia controls. Results: The expression of HMGA1 mRNA in 30 patients with acute leukemia were statistically higher than those in 25 non-leukemic controls;the expression levels between ALL and AML had no significant difference,the expression of HMGA1 mRNA in megakaryocytic leukemia was the highest in all groups.Conclusion: HMGA1 expression levels in ALs were strikingly higher than that in non-leukemias,and were the highest in M7.HMGA1 can be the new target of gene therapy and the differential diagnosis index of M7. |
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| Keywords: | SYBR Green |
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