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BMI-1在宫颈癌组织中的表达及意义
引用本文:刘蓓,王琼,范军,贺玉娟,付军,曾桃英.BMI-1在宫颈癌组织中的表达及意义[J].微循环学杂志,2010,20(3):20-22,26,F0003.
作者姓名:刘蓓  王琼  范军  贺玉娟  付军  曾桃英
作者单位:武汉科技大学附属天佑医院病理科,武汉,430064
摘    要:目的:通过检测人宫颈癌组织中BMI-1的表达,探讨其与宫颈癌临床病理特征的关系。方法:分别采用RT-PCR和Western blot方法检测BMI-1基因在宫颈癌细胞株CasKi中mRNA水平及蛋白质水平的表达,进一步采用免疫组化SP法检测57例宫颈癌组织中BMI-1蛋白的表达情况,并结合患者的临床病理资料分析与其相关性。结果:BMI-1在宫颈癌细胞CasKi中,在mRNA水平和蛋白质水平均有表达,免疫组织化学检测的57例宫颈癌标本中BMI-1蛋白强阳性表达23例(80.70%),且BMI-1的强阳性表达与宫颈癌的淋巴结转移及临床分期密切相关(P0.05),而与肿瘤大小、雌激素受体(ER)和孕激素受体(PR)等临床病理特征无显著性相关(P0.05)。结论:BMI-1蛋白在宫颈癌组织中的高表达与肿瘤进展和淋巴结转移相关,提示高表达BMI-1的宫颈癌具有高度转移潜能,同时预示BMI-1可能成为预测宫颈癌高度转移的新的分子标志物。

关 键 词:宫颈癌  BMI-1  免疫组织化学  肿瘤生物标志物

Expression and Significance of BMI-1 in Cervical Cancer
Abstract:Objective:To detect the expression of BMI-1 in cervical cancer and analyze the relationship of BMI-1 with the clinicopathological features of cervical cancer.Method:The level of BMI-1 expression in cervical cancer cell line CasKi was detected by RT-PCR and Western blot.The expression of BMI-1 protein in 57 specimens of cervical cancer was detected by immunohistochemistry to analyze the correlation of BMI-1 expression to clinicopathologic features of cervical cancer.Results:The BMI-1 expressed in the level of mRNA and protein in CasKi cell line.The intensive positive rate of BMI-1 in cervical cancer was 80.70%.The intensive expression of BMI-1 was positively correlated to clinical stage and lymph node metastasis(P<0.05),but not to tumor size,estrogen receptor and progesterone receptor et al.(P>0.05).Conclusion:The intensive expression of BMI-1 in cervical cancer is related to tumor progression and lymph node metastasis,suggesting a high BMI-1 expression of cervical cancer is highly metastatic potential,while BMI-1 indicates the forecast could be a high degree of transfer of new tumor biomarker.
Keywords:BMI-1
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