三维骨微管结构支架构造及体外培养研究

目的：三维骨微管结构支架构造方法研究以及成骨细胞复合后的体外培养，观察这种能够为细胞生长提供三维骨微管结构的支架对细胞贴附、生长、增殖以及分化的影响。方法：应用快速成形技术制造支架负型模具，在模具中填充CPC材料，待其固化后，去除模具，形成具有内部相互连通微管的三维支架。复合成骨细胞，进行体外培养。分别于第4d和14d取出样本，用扫描电镜观察细胞生长情况。结果：利用光固化快速成形技术间接构造所得三维支架，具有很好的三维立体结构。扫描电镜下观察，成骨细胞在三维支架表面和微管内贴附生长状况良好，并分泌大量基质。结论：三维骨微管结构支架的快速成形间接构造方法应用于骨组织工程中支架的构造是可行的，所构造的CPC支架结构能够使细胞在其表面和微管内生长、增殖和分化。

Study of Fabrication of Scaffolds with 3D Bone Microchannel Structure and Culture in vitro

Objective:To study the fabrication methods of scaffold with 3D bone microchannel structure and culture in vitro after seeding osteoblast. Observing osteoblastic growth,proliferation and differentiation in the 3D porous scaffolds. Method:The rapid prototyping(RP)technology was used to fabricate negative molds of scaffolds. CPC pasty was cast into the molds. Subsequently,the molds were fired out after CPC being hardened to obtain the scaffolds with 3D bone microchannels. Osteoblast cells were seeded and cultured in vitro. Samples were taken out at the 4~ th day and 14~ th day and observed using scanning electron microscope. Results: The scaffolds indirectly fabricated by Stereolithography RP technology obtained good 3D spatial structure. Osteoblast can attach and proliferate on the surface and in the micropore of scaffolds. Conclusion:It is feasible for indirectly fabricating 3D porous scaffolds using RP technology in the application of bone tissue engineering. The CPC scaffolds construct provided an acceptable scaffold for osteoblastic growth,proliferation and differentiation.