人结直肠癌组织中表皮生长因子受体1与Fascin-1蛋白表达的相关性及其临床意义

目的探讨人结直肠癌组织中表皮生长因子受体1(EGFR)与Fascin-1蛋白表达的关系及其临床意义。方法回顾性收集2008年1月—2010年12月东阳市人民医院病理科结直肠癌石蜡标本258例和同时切除的正常结直肠组织石蜡标本72例。采用免疫组织化学EnVision法检测并比较直肠癌组织和正常结直肠组织中EGFR和Fascin-1蛋白的表达;分析结直肠癌中Fascin-1蛋白表达与结直肠癌患者临床病理特征、EGFR的关系,以及EGFR和Fascin-1蛋白的表达与患者的预后生存情况的关系。结果(1)结直肠癌组织中Fascin-1蛋白的阳性率为38.0%(98/258),高于正常结直肠组织的阳性率0.0%(0/72),差异有统计学意义(χ2=38.901,P<0.01)。(2)女性、结肠及低分化的结直肠癌患者中Fascin-1蛋白的阳性率高于男性、直肠及高-中分化患者(χ2=4.256、20.085、8.471,P值均<0.05)。(3)在EGFR阳性结直肠癌患者的Fascin-1蛋白阳性表达率(42.9%,91/212)较EGFR阴性病例(15.2%,7/46)高,差异有统计学意义(χ2=12.318,P<0.01);Spearman相关分析显示,结直肠癌组织中EGFR和Fascin-1蛋白表达呈正相关(rs=0.219,P<0.01)。(4)194例获得随访的结直肠癌患者5年总生存率为73.2%(142/194),5年无复发生存率为65.5%(127/194)。其中EGFR和Fascin-1均阳性患者的5年平均总生存期及5年总生存率(47.8个月、64.7%)低于非均阳性患者(54.4个月、77.8%),差异有统计学意义(χ2=5.039,P<0.05)。结论结直肠癌中EGFR与Fascin-1蛋白的表达呈正相关,二者同时表达与患者预后不良有关。

Correlation and clinical significance of epidermal growth factor receptor 1 and Fascin-1 expression in colorectal cancer

Objective To investigate the relationship between epidermal growth factor receptor(EGFR) and Fascin-1 protein expression in colorectal cancer and the clinical significance.Methods Retrospectively collected 258 cases of colorectal cancer and 72 cases of normal colorectal tissue paraffin specimens from the pathology department of Dongyang People's Hospital from January 2008 to December 2010. Immunohistochemical EnVision method was used to detect the expression of EGFR and Fascin-1 protein in colorectal cancer tissues and normal colorectal tissues. Relationship between Fascin-1 and clinicopathological characteristics, EGFR and clinical prognostic significance in colorectal cancer were analyzed.Results The positive rate of Fascin-1 protein in normal colorectal tissues was 0.0% (0/72), while the positive rate in colorectal cancer was 38.0% (98/258). The positive rate of Fascin-1 protein in colorectal cancer was significantly higher than that in normal colorectal tissue (χ²=38.901, P＜0.01). The positive rate of Fascin-1 protein in women, colon and poorly differentiated colorectal cancer patients was significantly higher than that in male, rectal and high-middle differentiated patients (χ²=4.256, 20.085, 8.471, all P values＜0.05). In EGFR-positive colorectal cancer, the positive rate of Fascin-1 protein (42.9%, 91/212) was significantly higher than that of EGFR-negative cases (15.2%, 7/46) with statistically significant difference(χ²=12.318, P＜0.01). Spearman correlation analysis revealed a significant positive correlation between EGFR and Fascin-1 protein expression in colorectal cancer (r_s=0.219, P＜0.01). The 5-year overall survival rate of 194 followed up patients with colorectal cancer was 73.2% (142/194), and the 5-year recurrence-free survival rate was 65.5% (127/194). The 5-year mean overall survival and 5-year overall survival (47.8 months, 64.7%) of patients with positive EGFR and Fascin-1 were significantly lower than those of non-uniform positive patients (54.4 months, 77.8%), with statistically significant difference (χ²=5.039, P＜0.05).Conclusions There is a significantly positive correlation between EGFR and Fascin-1 expression in colorectal cancer, and their simultaneous expression is associated with poor prognosis.