氯吡格雷对人早期内皮祖细胞黏附、迁移及增殖功能的影响

目的探讨氯吡格雷对人早期内皮祖细胞(EPCs)黏附、迁移及增殖功能的影响。方法体外培养人外周血早期EPCs并进行鉴定;将含不同浓度(1×10-3～200×10-3mmol.L-1)氯吡格雷的培养液与EPCs共培养24 h,检测黏附、迁移及增殖功能;应用含浓度为20×10-3mmol.L-1氯吡格雷的培养液与EPCs共培养0.5～72.0 h,检测EPCs上述功能。结果培养EPCs第4天,早期EPCs呈典型长梭形;培养EPCs第7天数目增多,可摄取Dil标记的乙酰化低密度脂蛋白以及FITC标记的荆豆凝集素。氯吡格雷可使细胞明显增多;不同浓度的氯吡格雷可改善其黏附(F=56.54,P=0.00)、迁移(F=60.23,P=0.00)和增殖(F=1.45,P=0.16)功能;氯吡格雷浓度为20×10-3mmol.L-1时,保护作用最强;当共培养不同时间后,其仍可改善EPCs黏附(F=127.03,P=0.00)、迁移(F=96.03,P=0.00)和增殖(F=10.46,P=0.00)功能;保护作用呈时间依赖性,但于24 h后达到平台期。结论氯吡格雷可改善人外周血早期EPCs的黏附、迁移及增殖功能,且具有浓度依赖性和时间依赖性。

Effects of clopidogrel on the adherence,migration and proliferation of early endothelial progenitor cells from human peripheral blood

Objective To evaluate the effect of clopidogrel on the adherence,migration and proliferation of early endothelial progenitor cells(EPCs) from human peripheral blood. Methods Early EPCs were cultured and characterized.Then,EPCs were cultured for 24 hours with medium containing clopidogrel of different concentrations(1×10-3-200×10-3 mmol·L-1),and the adherence,migration and proliferation were tested;furthermore,the EPCs were cultured with medium containing 20×10-3 mmol·L-1 clopidogrel for different time(0.5-72.0 h),and analysed the adherence,migration and proliferation again. Results The EPCs became long spindle on the 4th day,and these cells increased on the 7th day.Moreover,EPCs could take in Dil-acetylated low density lipoprotein(Dil-acLDL) and FITC-ulex europaeus agglutinin(FITC-UEA-I).Clopidogrel could significantly increase the number of cell;the different concentrations of clopidogrel could improve the function of adhesion(F=56.54,P=0.00),migration(F=60.23,P=0.00) and proliferation(F=1.45,P=0.16).The protection was highest when the clopidogrel was 20×10-3 mmol·L-1.The result showed that 20×10-3 mmol·L-1 clopidogrel could improve EPCs adherence(F=127.03,P=0.00),migration(F=96.03,P=0.00) and proliferation(F=10.46,P=0.00),and the protective effect was time-dependent,but did not changed significantly after 24 hours. Conclusion Clopidogrel can improve the adherence,migration and proliferation function of early EPCs from human peripheral blood in concentration-and time-dependent manner.