腰椎间盘退变动物模型的建立

目的　建立腰椎间盘退变的动物模型并行组织学和MRI观察。　方法　健康成年恒河猴 8只 ,手术组 6只 ,在其腰4 ,5、腰5,6、腰6,7椎间盘分别作一 5mm× 3mm切口 ,分别于术前及术后 1、4、8、13、2 0周对手术椎间盘行MRI观察 ;8只恒河猴分别于术前 (对照组 )及术后 4、8、2 0周随机取 2只处死后行椎间盘组织学观察。　结果　正常成年恒河猴腰椎间盘组织学结构特点与人类相似 ;手术椎间盘高信号区域面积的百分比于术后 8、13、2 0周显著减少 (P <0 0 5 ) ;术后 4周椎间盘切口外被纤维组织覆盖 ,术后 8周 ,切口外层纤维样愈合 ,其内无愈合迹象 ,术后 2 0周 ,切口向椎间盘内延伸 ,部分髓核出现纤维化。　结论　恒河猴是一种生理特性与人类相接近的动物 ,以其为对象应用纤维环切开法可以获得可靠的腰椎间盘退变动物模型 ;通过测量MRI轴位T2加权像椎间盘高信号区域面积百分比的方法可于早期无损伤地发现椎间盘退变。

The development of degenerative disc animal model in rhesus monkey

OBJECTIVE: To develop the degenerative disc animal model with histological and MRI observation. METHODS: Eight adult Rhesus monkeys were divided randomly into two groups after the lumbar discs MRI scan. The operated group, 6 monkeys, was subjected to annulus resection. A 5 mm x 3 mm cut was made in the left anterolateral part of the fibrosus annulus on L(4,5), L(5,6) and L(6,7) discs. The operated discs were scanned with MRI at 1 week, 4 weeks, 8 weeks, 13 weeks and 20 weeks postoperatively and observed histologically at 4 weeks, 8 weeks and 20 weeks after operation. RESULTS: The histological structure of Rhesus monkey was similar to that of human being. The percentage of high signal intensity area in the operated discs reduced significantly at 8, 13, 20 weeks after operation (P < 0.05). The fibroplasia outside the annular tear could be observed in all 6 operated discs at 4 weeks postoperatively. Two of 6 operated discs underwent fibroplasia at 20 weeks after operation. No heal evidence in the inner tear was showed after operation. CONCLUSIONS: The animal model for human lumbar disc degeneration could be obtained by anterio-lateral annulus resection. The early-stage degenerative evidence could be observed without injury by measuring the percentage of the high intensity area of the operated discs on T2WI of MRI.